A PCR-based screening program to assess the prevalence of Taylorella equigenitalis in breeding stallions in South Africa

Abstract

The first outbreak of Contagious Equine Metritis (CEM) due to Taylorella equigenitalis in South Africa was reported to the OIE in May 2011 subsequent to importation of a stallion, the index case. Two additional positive stallions were identified on an initial trace-back. The outbreak-response prompted determination of the national prevalence and distribution of CEM. A nation-wide PCR-based screening of all breeding stallions motivated by a previous outbreak report [1] was implemented via a mandatory CEM-negative clearance certificate prior to use for natural breeding or semen collection. Compliance from breeders was facilitated by developing a web-based system providing an easily-accessed, rapid and costeffective sampling, testing and reporting process on www.cemsa.co.za. A submission form, information, a breed-indexed list of stallions achieving CEM-clearance and a method for obtaining and submitting two sets of swabs (with an interval > 7d) from the external genitalia were accessible on the website. A duplex PCR was chosen as the assay method due to potential for submission of samples with minimal restrictions on transit time and temperature criteria and rapid, high throughput, cost-effi-ciency and reported sensitivity *1,2+. A clearance certificate was issued via the website after negative results from both sets of samples.