Serological and molecular investigation of Newcastle disease in household chicken flocks and associated markets in Eastern Shewa zone, Ethiopia

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dc.contributor.author Chaka, Hassen
dc.contributor.author Goutard, Flavie
dc.contributor.author Gil, Patricia
dc.contributor.author Abolnik, Celia
dc.contributor.author De Almeida, Renata Servan
dc.contributor.author Bisschop, S.P.R. (Shahn)
dc.contributor.author Thompson, P.N. (Peter N.)
dc.date.accessioned 2016-10-27T10:47:24Z
dc.date.available 2016-10-27T10:47:24Z
dc.date.issued 2013-03
dc.description.abstract Cross-sectional survey for Newcastle disease (ND) were conducted in nonvaccinated household flocks of village chickens to assess serological and virological ND status in households and associated live bird markets. In total, 1,899 sera and 460 pools of cloacal and tracheal swabs were sampled and tested using a commercial enzyme-linked immunosorbent assay (ELISA) and real-time reverse transcriptase polymerase chain reaction (rRT-PCR), respectively. Additionally, paired cloacal and tracheal swabs from 1,269 individual chickens were collected from markets and tested using RT-PCR. The prevalence of households with at least one seropositive chicken was higher during the dry season (27.4 %) than during the wet season (17.4 %) (P = 0.003). Viral genome was detected in 14.2 % of households during the wet season using a fusion (F) gene assay and in 24.2 % of households during the dry season using a polymerase (L) gene assay that targets both class I and class II viruses. At the markets sampled, overall bird level prevalence was 4.9 % for period 1 (F gene assay), and 38.2 % and 27.6 % for periods 2 and 3, respectively (L gene assay). Partial sequencing of the F gene (239 bp) cleavage site indicated that the majority of the circulating strains exhibited motifs specific to virulent strains. Seroepidemiology coupled with molecular analysis can be a useful tool to assess the status of NDV infection. The village chicken population in Ethiopia is endemically infected with virulent NDV that pose a significant threat to emerging small- and medium-scale commercial poultry production. en_ZA
dc.description.department Production Animal Studies en_ZA
dc.description.librarian hb2016 en_ZA
dc.description.sponsorship The French Ministry of Foreign and European Affairs (MAEE). en_ZA
dc.description.uri http://link.springer.com/journal/11250 en_ZA
dc.identifier.citation Chaka, H., Goutard, F., Gil, P., Abolnik, C., De Almeida, R.S., Bisschop, S. & Thompson, P.N. Serological and molecular investigation of Newcastle disease in household chicken flocks and associated markets in Eastern Shewa zone, Ethiopia. Tropical Animal Health and Production (2013) 45: 705-714. doi:10.1007/s11250-012-0278-y en_ZA
dc.identifier.issn 0049-4747 (print)
dc.identifier.issn 1573-7438 (online)
dc.identifier.other 10.1007/s11250-012-0278-y
dc.identifier.other 23093208700
dc.identifier.other N-9324-2014
dc.identifier.other 7403220452
dc.identifier.other J-2534-2013
dc.identifier.uri http://hdl.handle.net/2263/57530
dc.language.iso en en_ZA
dc.publisher Springer en_ZA
dc.rights © Springer Science+Business Media Dordrecht 2012. The original publication is available at : http://link.springer.comjournal/11250. en_ZA
dc.subject Village chickens en_ZA
dc.subject Ethiopia en_ZA
dc.subject Newcastle disease (ND) en_ZA
dc.subject Enzyme-linked immunosorbent assay (ELISA) en_ZA
dc.subject Real-time reverse transcriptase polymerase chain reaction (rRT-PCR) en_ZA
dc.title Serological and molecular investigation of Newcastle disease in household chicken flocks and associated markets in Eastern Shewa zone, Ethiopia en_ZA
dc.type Postprint Article en_ZA


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