dc.contributor.author |
Thi Le, Phuong
|
|
dc.contributor.author |
Makhalanyane, Thulani P.
|
|
dc.contributor.author |
Guerrero, Leandro D.
|
|
dc.contributor.author |
Vikram, Surendra
|
|
dc.contributor.author |
Van de Peer, Yves
|
|
dc.contributor.author |
Cowan, Don A.
|
|
dc.date.accessioned |
2016-10-03T11:19:33Z |
|
dc.date.available |
2016-10-03T11:19:33Z |
|
dc.date.issued |
2016 |
|
dc.description.abstract |
Understanding microbial adaptation to environmental stressors is crucial for interpreting broader ecological patterns. In the most
extreme hot and cold deserts, cryptic niche communities are thought to play key roles in ecosystem processes and represent excellent
model systems for investigating microbial responses to environmental stressors. However, relatively little is known about the genetic
diversity underlying such functional processes in climatically extreme desert systems. This study presents the first comparative
metagenome analysis of cyanobacteria-dominated hypolithic communities in hot (Namib Desert, Namibia) and cold (Miers Valley,
Antarctica) hyperarid deserts. The most abundant phyla in both hypolith metagenomes were Actinobacteria, Proteobacteria,
Cyanobacteria and Bacteroidetes with Cyanobacteria dominating in Antarctic hypoliths. However, no significant differences between
the twometagenomeswere identified. The Antarctic hypolithicmetagenome displayed a high number of sequences assigned
to sigma factors, replication,recombination andrepair, translation, ribosomal structure,andbiogenesis. In contrast, theNamibDesert
metagenome showed a high abundance of sequences assigned to carbohydrate transport and metabolism. Metagenome data
analysis also revealed significantdivergence inthe geneticdeterminantsof aminoacidandnucleotidemetabolismbetween these two
metagenomes and those of soil from other polar deserts, hot deserts, and non-desert soils. Our results suggest extensive niche
differentiation in hypolithic microbial communities from these two extreme environments and a high genetic capacity for survival
under environmental extremes. |
en_ZA |
dc.description.department |
Genetics |
en_ZA |
dc.description.librarian |
am2016 |
en_ZA |
dc.description.sponsorship |
The authors gratefully acknowledge the following organizations:
The University of Pretoria Genomics Research Institute
(PTL, TPM, DAC, and YVdP), South African National Antarctic
Program (SANAP) and the National Research Foundation (NRF)
for funding (LG, SV). |
en_ZA |
dc.description.uri |
http://gbe.oxfordjournals.org |
en_ZA |
dc.identifier.citation |
Thi Le, P, Makhalanyane, TP, Guerrero, LD, Vikram, S, Van de Peer, Y & Cowan, DA 2016, 'Comparative metagenomic analysis reveals mechanisms for stress response in hypoliths from extreme hyperarid deserts', Genome Biology and Evolution, vol. 8, no. 9, pp. 2737-2747. |
en_ZA |
dc.identifier.issn |
1759-6653 |
|
dc.identifier.other |
10.1093/gbe/evw189 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/57076 |
|
dc.language.iso |
en |
en_ZA |
dc.publisher |
Oxford University Press |
en_ZA |
dc.rights |
The Author(s) 2016. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License. |
en_ZA |
dc.subject |
Stress response |
en_ZA |
dc.subject |
Deserts |
en_ZA |
dc.subject |
Comparative metagenomics |
en_ZA |
dc.subject |
Antarctica |
en_ZA |
dc.subject |
Namib Desert |
en_ZA |
dc.subject |
Hypoliths |
en_ZA |
dc.subject |
Soils |
en_ZA |
dc.subject |
Biomes |
en_ZA |
dc.title |
Comparative metagenomic analysis reveals mechanisms for stress response in hypoliths from extreme hyperarid deserts |
en_ZA |
dc.type |
Article |
en_ZA |