Comparative metagenomic analysis reveals mechanisms for stress response in hypoliths from extreme hyperarid deserts

Show simple item record Thi Le, Phuong Makhalanyane, Thulani P. Guerrero, Leandro D. Vikram, Surendra Van de Peer, Yves Cowan, Don A. 2016-10-03T11:19:33Z 2016-10-03T11:19:33Z 2016
dc.description.abstract Understanding microbial adaptation to environmental stressors is crucial for interpreting broader ecological patterns. In the most extreme hot and cold deserts, cryptic niche communities are thought to play key roles in ecosystem processes and represent excellent model systems for investigating microbial responses to environmental stressors. However, relatively little is known about the genetic diversity underlying such functional processes in climatically extreme desert systems. This study presents the first comparative metagenome analysis of cyanobacteria-dominated hypolithic communities in hot (Namib Desert, Namibia) and cold (Miers Valley, Antarctica) hyperarid deserts. The most abundant phyla in both hypolith metagenomes were Actinobacteria, Proteobacteria, Cyanobacteria and Bacteroidetes with Cyanobacteria dominating in Antarctic hypoliths. However, no significant differences between the twometagenomeswere identified. The Antarctic hypolithicmetagenome displayed a high number of sequences assigned to sigma factors, replication,recombination andrepair, translation, ribosomal structure,andbiogenesis. In contrast, theNamibDesert metagenome showed a high abundance of sequences assigned to carbohydrate transport and metabolism. Metagenome data analysis also revealed significantdivergence inthe geneticdeterminantsof aminoacidandnucleotidemetabolismbetween these two metagenomes and those of soil from other polar deserts, hot deserts, and non-desert soils. Our results suggest extensive niche differentiation in hypolithic microbial communities from these two extreme environments and a high genetic capacity for survival under environmental extremes. en_ZA
dc.description.department Genetics en_ZA
dc.description.librarian am2016 en_ZA
dc.description.sponsorship The authors gratefully acknowledge the following organizations: The University of Pretoria Genomics Research Institute (PTL, TPM, DAC, and YVdP), South African National Antarctic Program (SANAP) and the National Research Foundation (NRF) for funding (LG, SV). en_ZA
dc.description.uri en_ZA
dc.identifier.citation Thi Le, P, Makhalanyane, TP, Guerrero, LD, Vikram, S, Van de Peer, Y & Cowan, DA 2016, 'Comparative metagenomic analysis reveals mechanisms for stress response in hypoliths from extreme hyperarid deserts', Genome Biology and Evolution, vol. 8, no. 9, pp. 2737-2747. en_ZA
dc.identifier.issn 1759-6653
dc.identifier.other 10.1093/gbe/evw189
dc.language.iso en en_ZA
dc.publisher Oxford University Press en_ZA
dc.rights The Author(s) 2016. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License. en_ZA
dc.subject Stress response en_ZA
dc.subject Deserts en_ZA
dc.subject Comparative metagenomics en_ZA
dc.subject Antarctica en_ZA
dc.subject Namib Desert en_ZA
dc.subject Hypoliths en_ZA
dc.subject Soils en_ZA
dc.subject Biomes en_ZA
dc.title Comparative metagenomic analysis reveals mechanisms for stress response in hypoliths from extreme hyperarid deserts en_ZA
dc.type Article en_ZA

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