Investigating the diversity of the 18S SSU rRNA hyper-variable region of Theileria in cattle and Cape buffalo (Syncerus caffer) from southern Africa using a next generation sequencing approach

Show simple item record

dc.contributor.author Mans, Ben J. (Barend Johannes)
dc.contributor.author Pienaar, Ronel
dc.contributor.author Ratabane, John
dc.contributor.author Pule, Boitumelo
dc.contributor.author Latif, Abdalla A.
dc.date.accessioned 2016-09-22T08:04:19Z
dc.date.issued 2016-07
dc.description.abstract Molecular classification and systematics of the Theileria is based on the analysis of the 18S rRNA gene. Reverse line blot or conventional sequencing approaches have disadvantages in the study of 18S rRNA diversity and a next-generation 454 sequencing approach was investigated. The 18S rRNA gene was amplified using RLB primers coupled to 96 unique sequence identifiers (MIDs). Theileria positive samples from African buffalo (672) and cattle (480) from southern Africa were combined in batches of 96 and sequenced using the GS Junior 454 sequencer to produce 825711 informative sequences. Sequences were extracted based on MIDs and analysed to identify Theileria genotypes. Genotypes observed in buffalo and cattle were confirmed in the current study, while no new genotypes were discovered. Genotypes showed specific geographic distributions, most probably linked with vector distributions. Host specificity of buffalo and cattle specific genotypes were confirmed and prevalence data as well as relative parasitemia trends indicate preference for different hosts. Mixed infections are common with African buffalo carrying more genotypes compared to cattle. Associative or exclusion co-infection profiles were observed between genotypes that may have implications for speciation and systematics: specifically that more Theileria species may exist in cattle and buffalo than currently recognized. Analysis of primers used for Theileria parva diagnostics indicate that no new genotypes will be amplified by the current primer sets confirming their specificity. T. parva SNP variants that occur in the 18S rRNA hypervariable region were confirmed. A next generation sequencing approach is useful in obtaining comprehensive knowledge regarding 18S rRNA diversity and prevalence for the Theileria, allowing for the assessment of systematics and diagnostic assays based on the 18S gene. en_ZA
dc.description.department Veterinary Tropical Diseases en_ZA
dc.description.embargo 2017-07-31
dc.description.librarian hb2016 en_ZA
dc.description.sponsorship The Theileria diagnostics project ARC-OVI (OV15/08/1P01) and the Department of Agriculture, Forestry and Fisheries (OV21/03/C148). en_ZA
dc.description.uri http://www.elsevier.com/locate/ttbdis en_ZA
dc.identifier.citation Mans, B, Pienaar, R, Ratabane, J, Pule, B & Latif, AA 2016, 'Investigating the diversity of the 18S SSU rRNA hyper-variable region of Theileria in cattle and Cape buffalo (Syncerus caffer) from southern Africa using a next generation sequencing approach', Ticks and Tick-borne Diseases, vol. 7, no. 5, pp. 869-879. en_ZA
dc.identifier.issn 1877-959X ( print)
dc.identifier.issn 1877-9603 (online)
dc.identifier.other 10.1016/j.ttbdis.2016.04.005
dc.identifier.uri http://hdl.handle.net/2263/56878
dc.language.iso en en_ZA
dc.publisher Elsevier en_ZA
dc.rights © 2016 Elsevier GmbH. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Ticks and Tick-borne Diseases. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in Ticks and Tick-borne Diseases, vol.7, no. 5, pp. 869-879, 2016. doi : 10.1016/j.ttbdis.2016.04.005. en_ZA
dc.subject 18S SSU en_ZA
dc.subject Diversity en_ZA
dc.subject Theileria en_ZA
dc.subject Theileria parva en_ZA
dc.subject Cape buffalo en_ZA
dc.subject Cattle en_ZA
dc.subject Next generation sequencing (NGS) en_ZA
dc.subject Syncerus caffer
dc.title Investigating the diversity of the 18S SSU rRNA hyper-variable region of Theileria in cattle and Cape buffalo (Syncerus caffer) from southern Africa using a next generation sequencing approach en_ZA
dc.type Postprint Article en_ZA


Files in this item

This item appears in the following Collection(s)

Show simple item record