The role of the human microbiome on cancer progression remains unclear. Therefore, in
this study, we investigated the influence of some quorum sensing peptides, produced by diverse
commensal or pathogenic bacteria, on breast cancer cell invasion and thus cancer
outcome. Based on microscopy, transcriptome and Chick Chorioallantoic Membrane
(CAM) analyses, four peptides (PhrG from B. subtilis, CSP from S. mitis and EDF from
E. coli, together with its tripeptide analogue) were found to promote tumour cell invasion
and angiogenesis, thereby potentially influencing tumour metastasis. Our results offer not
only new insights on the possible role of the microbiome, but also further opportunities in
cancer prevention and therapy by competing with these endogenous molecules and/or by
modifying people’s life style.
S1 Table. List of quorum sensing peptides, repeatedly (n = 3) investigated for their effect
on tumour cell invasion (Collagen Type 1 invasion assay).