dc.contributor.author |
Maree, Sonja
|
|
dc.contributor.author |
Maree, Francois Frederick
|
|
dc.contributor.author |
Putterill, John F.
|
|
dc.contributor.author |
De Beer, Tjaart A.P.
|
|
dc.contributor.author |
Huismans, H. (Henk), 1942-
|
|
dc.contributor.author |
Theron, Jacques
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|
dc.date.accessioned |
2016-09-12T12:48:01Z |
|
dc.date.issued |
2016-02 |
|
dc.description.abstract |
As a means to develop African horse sickness (AHS) vaccines that are safe and DIVA compliant, we investigated the synthesis of empty African horse sickness virus (AHSV) particles. The emphasis of this study was on the assembly of the major viral core (VP3 and VP7) and outer capsid proteins (VP2 and VP5) into architecturally complex, heteromultimeric nanosized particles. The production of fully assembled core-like particles (CLPs) was accomplished in vivo by baculovirus-mediated co-synthesis of VP3 and VP7. The two different outer capsid proteins were capable of associating independently of each other with preformed cores to yield partial virus-like particles (VLPs). Complete VLPs were synthesized, albeit with a low yield. Crystalline formation of AHSV VP7 trimers is thought to impede high-level CLP production. Consequently, we engineered and co-synthesized VP3 with a more hydrophilic mutant VP7, resulting in an increase in the turnover of CLPs. |
en_ZA |
dc.description.department |
Genetics |
en_ZA |
dc.description.department |
Microbiology and Plant Pathology |
en_ZA |
dc.description.embargo |
2017-02-28 |
|
dc.description.librarian |
hb2016 |
en_ZA |
dc.description.sponsorship |
The Agricultural Research Council (ARC) and National
Research Foundation (NRF). |
en_ZA |
dc.description.uri |
http://www.elsevier.com/locate/virusres |
en_ZA |
dc.identifier.citation |
Maree, S, Maree, FF, Putterill, JF, De Beer, TAP, Huismans, H & Theron, J 2016, 'Synthesis of empty African horse sickness virus particles', Virus Research, vol. 213, pp. 184-194. |
en_ZA |
dc.identifier.issn |
0168-1702 (print) |
|
dc.identifier.issn |
1872-7492 (online) |
|
dc.identifier.other |
10.1016/j.virusres.2015.12.006 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/56715 |
|
dc.language.iso |
en |
en_ZA |
dc.publisher |
Elsevier |
en_ZA |
dc.rights |
© 2015 Elsevier B.V. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Virus Research. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in Virus Research, vol. 213, pp.184-194, 2016. doi : 10.1016/j.virusres.2015.12.006. |
en_ZA |
dc.subject |
African horse sickness (AHS) |
en_ZA |
dc.subject |
African horse sickness virus (AHSV) |
en_ZA |
dc.subject |
Core-like particles (CLPs) |
en_ZA |
dc.subject |
Virus-like particles (VLPs) |
en_ZA |
dc.title |
Synthesis of empty African horse sickness virus particles |
en_ZA |
dc.type |
Postprint Article |
en_ZA |