dc.contributor.author |
Nemesio-Gorriz, Miguel
|
|
dc.contributor.author |
Hammerbacher, Almuth
|
|
dc.contributor.author |
Ihrmark, Katarina
|
|
dc.contributor.author |
Källman, Thomas
|
|
dc.contributor.author |
Olson, Åke
|
|
dc.contributor.author |
Lascoux, Martin
|
|
dc.contributor.author |
Stenlid, Jan
|
|
dc.contributor.author |
Gershenzon, Jonathan
|
|
dc.contributor.author |
Elfstrand, Malin
|
|
dc.date.accessioned |
2016-09-12T12:30:21Z |
|
dc.date.available |
2016-09-12T12:30:21Z |
|
dc.date.issued |
2016-08 |
|
dc.description.abstract |
Despite the fact that fungal diseases are a growing menace for conifers in
modern silviculture, only a very limited number of molecular markers for
pathogen resistance have been validated in conifer species. A previous genetic
study indicated that the resistance of Norway spruce (Picea abies) to
Heterobasidion annosum s.l., a pathogenic basidiomycete species complex, is
linked to a QTL that associates with differences in fungal growth in sapwood
(FGS) that includes a gene, PaLAR3, which encodes a leucoanthocyanidin
reductase. In this study, gene sequences showed the presence of two PaLAR3
allelic lineages in P. abies. Higher resistance was associated with the novel allele,
which was found in low frequency in the four P. abies populations that we
studied. Norway spruce plants carrying at least one copy of the novel allele
showed a significant reduction in FGS after inoculation with Heterobasidion
parviporum compared to their half-siblings carrying no copies, indicating
dominance of this allele. The amount of (+) catechin, the enzymatic product of
PaLAR3, was significantly higher in bark of trees homozygous for the novel allele.
Although we observed that the in vitro activities of the enzymes encoded by the
two alleles were similar, we could show that allele-specific transcript levels were
significantly higher for the novel allele, indicating that regulation of gene
expression is responsible for the observed effects in resistance, possibly caused by differences in cis-acting elements that we observe in the promoter region of
the two alleles. |
en_ZA |
dc.description.department |
Forestry and Agricultural Biotechnology Institute (FABI) |
en_ZA |
dc.description.department |
Microbiology and Plant Pathology |
en_ZA |
dc.description.librarian |
hb2016 |
en_ZA |
dc.description.sponsorship |
Financial support was received from SSF (grant nr R8b08-0011), and by the
Swedish Research Council FORMAS, grant nrs 2012-1276 and 217-2007-433, the
European Community’s Sixth Framework Programme, under the Network of
Excellence Evoltree (www.evoltree.eu/) and by the Seventh Framework Programme
(FP7/2007-2013) under the grant agreement nu 211868 (Project Noveltree,
www.noveltree.eu/), Support also came from the Biodiversa projects Linktree
(www.igv.fi.cnr.it/linktree/) and TipTree, and The Max Planck Society. |
en_ZA |
dc.description.uri |
http://www.aspbjournals.org |
en_ZA |
dc.identifier.citation |
Nemesio-Gorriz, M, Hammerbacher, A, Ihrmark, K, Källman, T, Olson, Å, Lascoux, M, Stenlid, J, Gershenzon, J & Elfstrand, M 2016, 'Different alleles of a gene encoding leucoanthocyanidin reductase (PaLAR3) influence resistance against the fungus Heterobasidion parviporum in Picea abies', Plant Physiology, vol. 171, no. 4, pp. 2671-2681. |
en_ZA |
dc.identifier.issn |
0032-0889 (print) |
|
dc.identifier.issn |
1532-2548 (online) |
|
dc.identifier.other |
10.1104/pp.16.00685 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/56714 |
|
dc.language.iso |
en |
en_ZA |
dc.publisher |
American Society of Plant Biologists |
en_ZA |
dc.rights |
© 2016 American Society of Plant Biologists. All rights reserved. |
en_ZA |
dc.subject |
Norway spruce |
en_ZA |
dc.subject |
Picea abies |
en_ZA |
dc.subject |
Heterobasidion |
en_ZA |
dc.subject |
Catechin |
en_ZA |
dc.subject |
Leucoanthocyanidin reductase (LAR) |
en_ZA |
dc.subject |
Flavonoid |
en_ZA |
dc.subject |
Resistance |
en_ZA |
dc.subject |
Allele |
en_ZA |
dc.subject |
Quantitative trait loci (QTL) |
en_ZA |
dc.title |
Different alleles of a gene encoding leucoanthocyanidin reductase (PaLAR3) influence resistance against the fungus Heterobasidion parviporum in Picea abies |
en_ZA |
dc.type |
Postprint Article |
en_ZA |