dc.contributor.author |
Thaver, Veneesha
|
|
dc.contributor.author |
Meyer, Jacobus Johannes Marion
|
|
dc.contributor.author |
Cockeran, Riana
|
|
dc.contributor.author |
Cholo, Moloko C.
|
|
dc.contributor.author |
Anderson, Ronald
|
|
dc.contributor.author |
Lall, Namrita
|
|
dc.date.accessioned |
2016-09-07T07:18:51Z |
|
dc.date.available |
2016-09-07T07:18:51Z |
|
dc.date.issued |
2012-05 |
|
dc.description.abstract |
Although the naphthoquinone, 7-methyljuglone (7-MJ), is active against Mycobacterium tuberculosis (MTB) in vitro, neither the cellular site nor mechanism of anti-mycobacterial action of this agent has been identified. The primary objective of the current study was to investigate the mycobacterial outer membrane as a potential target of 7-MJ by measuring the effects of this agent (0.023 - 1.5 mg/L) on microbial ATP levels and uptake of K+. Methods: Bioluminescence and radiometric (uptake of 86Rb+) procedures were used to assay microbial ATP levels and K+ trans-port respectively. Results: Exposure of MTB (strain H37Rv) to 7-MJ for 60 min resulted in dose-related decreases in both microbial ATP levels and uptake of 86Rb+ which achieved statistical significance (P < 0.05) at concentrations of 0.4 and 0.1 mg/L respectively. Conclusions: These observations are compatible with the mycobacterial membrane as being the putative site of action of 7-MJ, targeting microbial energy metabolism and K+ transport. |
en_ZA |
dc.description.department |
Immunology |
en_ZA |
dc.description.department |
Plant Science |
en_ZA |
dc.description.librarian |
am2016 |
en_ZA |
dc.description.sponsorship |
The National Research Foundation and the Medical Research Council, Pretoria, South Africa. |
en_ZA |
dc.description.uri |
http://www.SciRP.org/journal/ojrd) |
en_ZA |
dc.identifier.citation |
Thaver, V, Meyer, JJM, Cockeran, R, Cholo, MC, Anderson, R & Lall, N 2012, 'Investigation of the anti-mycobacterial mechanism of action of 7-methyljuglone', Open Journal of Respiratory Diseases, vol. 2, no. 2, pp. 60-62. |
en_ZA |
dc.identifier.issn |
2163-940X (print) |
|
dc.identifier.issn |
2163-9418 (online) |
|
dc.identifier.other |
10.4236/ojrd.2012.22009 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/56642 |
|
dc.language.iso |
en |
en_ZA |
dc.publisher |
Scientific Research Publishing |
en_ZA |
dc.rights |
© 2012 SciRes |
en_ZA |
dc.subject |
Adenosine triphosphate |
en_ZA |
dc.subject |
Cell membrane |
en_ZA |
dc.subject |
Energy metabolism |
en_ZA |
dc.subject |
Potassium |
en_ZA |
dc.subject |
Mycobacterium tuberculosis (MTB) |
en_ZA |
dc.title |
Investigation of the anti-mycobacterial mechanism of action of 7-methyljuglone |
en_ZA |
dc.type |
Article |
en_ZA |