Identification and characterisation of oil sludge degrading bacteria isolated from compost

Show simple item record Ubani, Onyedikachi Atagana, Harrison Ifeanyichukwu Thantsha, Mapitsi Silvester Rasheed, Adeleke 2016-08-04T08:44:13Z 2016-08-04T08:44:13Z 2016
dc.description.abstract Compounds present in oil sludge such as polycyclic aromatic hydrocarbons (PAHs) are known to be cytotoxic, mutagenic and potentially carcinogenic. Microorganisms including bacteria and fungi have been reported to degrade oil sludge components to innocuous compounds such as carbon dioxide, water and salts. In the present study, we isolated different bacteria with PAH-degrading capabilities from compost prepared from oil sludge and animal manures. These bacteria were isolated on a mineral base medium and mineral salt agar plates. A total of 31 morphologically distinct isolates were carefully selected from 5 different compost treatments for identifi cation using polymerase chain reaction (PCR) of the 16S rRNA gene with specifi c primers (universal forward 16S-P1 PCR and reverse 16S-P2 PCR). The amplicons were sequenced and sequences were compared with the known nucleotides from the GenBank. The phylogenetic analyses of the isolates showed that they belong to 3 different clades; Firmicutes, Proteobacteria and Actinobacteria. These bacteria identifi ed were closely related to the genera Bacillus, Arthrobacter, Staphylococcus, Brevibacterium, Variovorax, Paenibacillus, Ralstonia and Geobacillus. The results showed that Bacillus species were predominant in all composts. Based on the results of the degradation of the PAHs in the composts and results of previous studies on bacterial degradation of hydrocarbons in oil, the characteristics of these bacterial isolates suggests that they may be responsible for the breakdown of PAHs of different molecular weights in the composts. Thus, they may be potentially useful for bioremediation of oil sludge during compost bioremediation. en_ZA
dc.description.department Microbiology and Plant Pathology en_ZA
dc.description.librarian am2016 en_ZA
dc.description.sponsorship The South African National Research Foundation en_ZA
dc.description.uri en_ZA
dc.identifier.citation Ubani, O, Atagana, HI, Thantsha, MS & Rasheed, A 2016, 'Identification and characterisation of oil sludge degrading bacteria isolated from compost', Archives of Environmental Protection, vol. 42, no. 2, pp. 67-77. en_ZA
dc.identifier.issn 2083-4772
dc.identifier.other 10.1515/aep-2016-0021
dc.language.iso en en_ZA
dc.publisher De Gruyter Open en_ZA
dc.rights © by Polish Academy of Sciences and Institute of Environmental Engineering of the Polish Academy of Sciences, Zabrze, Poland 2016 en_ZA
dc.subject Bioaugmentation en_ZA
dc.subject Biodegradation en_ZA
dc.subject Bioremediation en_ZA
dc.subject Composting en_ZA
dc.subject Oil sludge en_ZA
dc.subject Animal manures en_ZA
dc.subject Polycyclic aromatic hydrocarbons (PAHs) en_ZA
dc.subject Polymerase chain reaction (PCR) en_ZA
dc.title Identification and characterisation of oil sludge degrading bacteria isolated from compost en_ZA
dc.type Article en_ZA

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