Non-invasive measurement of adrenocortical activity in a myrmecophageous mammal, the bat-eared fox

Show simple item record Le Roux, A. Botha, H. Dalerum, Fredrik Ganswindt, Andre 2016-07-22T08:38:21Z 2016-02
dc.description.abstract Measuring physiological stress reactions through the quantification of plasma cortisol often involves physically restraint, which acts as a stressor itself. Here, we present the validation of a non-invasive method for assessing adrenocortical activity as an indicator of stress in the bat-eared fox (Otocyon megalotis). By conducting an adrenocorticotropic hormone (ACTH) challenge, we examined the suitability of three enzyme immunoassays (EIAs) detecting 11,17 dioxoandrostanes (11,17-DOA) as well as faecal glucocorticoid metabolites (fGCM) with a 5β-3α-ol-11-one (3α,11oxo-CM), or 11,17,21- trihydroxy-4-ene-20-one structure (cortisol), respectively, for monitoring stress-related physiological responses in male and female bat-eared foxes. Our results suggest that the cortisol EIA seems most suitable for measuring fGCMs in this myrmecophageous mammal. Using the cortisol EIA, we compared fGCM concentrations of three populations of foxes. Only one population experienced a sudden change in social environment and the assay appeared to effectively detect the resulting increase in stress hormone levels. Therefore, the identified EIA is effective at detecting intraspecific variation in fGCM levels, and hence a useful tool to evaluate physiological stress responses in this species. en_ZA
dc.description.department Anatomy and Physiology en_ZA
dc.description.department Mammal Research Institute en_ZA
dc.description.department Zoology and Entomology en_ZA
dc.description.embargo 2017-02-28
dc.description.librarian hb2016 en_ZA
dc.description.sponsorship Thuthuka grant (#TTK1206041007) from the National Research Foundation. en_ZA
dc.description.uri en_ZA
dc.identifier.citation Aliza Le Roux, Helene Botha, Fredrik Dalerum & Andre Ganswindt (2016)Non-invasive measurement of adrenocortical activity in a myrmecophageous mammal, the bat-eared fox, African Zoology, 51:1, 47-51 DOI: 10.1080/15627020.2016.1145071 en_ZA
dc.identifier.issn 1562-7020 (print)
dc.identifier.issn 2224-073X (online)
dc.identifier.other 10.1080/15627020.2016.1145071
dc.language.iso en en_ZA
dc.publisher Taylor and Francis and NISC (Pty) Ltd en_ZA
dc.rights © Zoological Society of Southern Africa. This is an electronic version of an article published in African Zoology, vol. 51, no.1, pp. 47-51, 2016. doi : 10.1080/15627020.2016.1145071. African Zoology is available online at : en_ZA
dc.subject Faecal glucocorticoid metabolite (fGCM) en_ZA
dc.subject Non-invasive hormone measurement en_ZA
dc.subject Otocyon megalotis en_ZA
dc.subject Stress en_ZA
dc.subject Adrenocorticotropic hormone (ACTH) en_ZA
dc.title Non-invasive measurement of adrenocortical activity in a myrmecophageous mammal, the bat-eared fox en_ZA
dc.type Postprint Article en_ZA

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