Infection of Ixodes spp. tick cells with different Anaplasma phagocytophilum isolates induces the inhibition of apoptotic cell death

Show simple item record Alberdi, Pilar Ayllón, Nieves Cabezas-Cruz, Alejandro Bell-Sakyi, Lesley Zweygarth, Erich Stuen, Snorre De la Fuente, José 2016-07-13T10:31:58Z 2015-09
dc.description.abstract Anaplasma phagocytophilum is an intracellular rickettsial pathogen transmitted by Ixodes spp. ticks, which causes granulocytic anaplasmosis in humans, horses and dogs and tick-borne fever (TBF) in ruminants. In the United States, human granulocytic anaplasmosis (HGA) is highly prevalent while TBF has not been reported. However, in Europe the situation is the opposite, with high prevalence for TBF in sheep and low prevalence of HGA. The origin of these differences has not been identified and our hypothesis is that different A. phagocytophilum isolates impact differently on tick vector capacity through inhibition of apoptosis to establish infection of the tick vector. In this study we used three different isolates of A. phagocytophilum of human, canine and ovine origin to infect the Ixodes ricinus-derived cell line IRE/CTVM20 and the Ixodes scapularis-derived cell line ISE6 in order to characterize the effect of infection on the level of tick cell apoptosis. Inhibition of apoptosis was observed by flow cytometry as early as 24 h post-infection for both tick cell lines and all three isolates of A. phagocytophilum, suggesting that pathogen infection inhibits apoptotic pathways to facilitate infection independently of the origin of the A. phagocytophilum isolate and tick vector species. However, infection with A. phagocytophilum isolates inhibited the intrinsic apoptosis pathway at different levels in I. scapularis and I. ricinus cells. These results suggested an impact of vector-pathogen co-evolution on the adaptation of A. phagocytophilum isolates to grow in tick cells as each isolate grew better in the tick cell line derived from its natural vector species. These results increase our understanding of the mechanisms of A. phagocytophilum infection and multiplication and suggest that multiple mechanisms may affect disease prevalence in different geographical regions. en_ZA
dc.description.department Veterinary Tropical Diseases en_ZA
dc.description.embargo 2016-09-30
dc.description.librarian hb2016 en_ZA
dc.description.sponsorship This research was supported by grants BFU2011-23896 and the EUFP7 ANTIGONE project number 278976. N. Ayllón was funded by MEC, Spain. A. Cabezas-Cruz was supported by a grant from the Ministère de l’Education Supérieure et de la Recherche of France.L. Bell-Sakyi was supported by the United Kingdom Biotechnology and Biological Sciences Research Council’s National Capability Grant to The Pirbright Institute. en_ZA
dc.description.uri en_ZA
dc.identifier.citation Alberdi, P, Ayllon, N, Cabezas-Cruz, A, Bell-Sakyi, L, Zweygarth, E, Stuen, S & De la Fuente, J 2015, 'Infection of Ixodes spp. tick cells with different Anaplasma phagocytophilum isolates induces the inhibition of apoptotic cell death', Ticks and Tick-borne Diseases, vol. 6, no. 6, pp. 758-767. en_ZA
dc.identifier.issn 1877-959X ( print)
dc.identifier.issn 1877-9603 (online)
dc.identifier.other 10.1016/j.ttbdis.2015.07.001
dc.language.iso en en_ZA
dc.publisher Elsevier en_ZA
dc.rights © 2015 Elsevier GmbH. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Ticks and Tick-borne Diseases. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Ticks and Tick-borne Diseases, vol. 6, no. 6, pp. 758-767, 2016. doi : 10.1016/j.ttbdis.2015.07.00. en_ZA
dc.subject Tick cell lines en_ZA
dc.subject Apoptosis en_ZA
dc.subject Porin en_ZA
dc.subject Anaplasma phagocytophilum en_ZA
dc.subject Ixodes en_ZA
dc.subject Tick-borne fever (TBF) en_ZA
dc.subject Human granulocytic anaplasmosis (HGA) en_ZA
dc.title Infection of Ixodes spp. tick cells with different Anaplasma phagocytophilum isolates induces the inhibition of apoptotic cell death en_ZA
dc.type Postprint Article en_ZA

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