Phagocytic activity of peritoneal exudate cells from mice

Show simple item record Hoogenhout, Joan Carter, P.
dc.contributor.editor De Lange, M.
dc.contributor.editor Bigalke, R.D.
dc.contributor.editor Howell, P.G.
dc.contributor.editor Reinecke, R.K.
dc.contributor.editor Walker, Jane B.
dc.contributor.other De Kock, V.E.
dc.contributor.upauthor Oellermann, R.A. 2016-07-07T07:44:18Z 2016-07-07T07:44:18Z 2016 1972
dc.description The articles have been scanned in colour with a HP Scanjet 5590;300dpi. adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format. en_ZA
dc.description.abstract Phagocytic activity of peritoneal exudate cells in mice was stimulated by injection of 0,1 ml mineral oil or varying concentrations of lysolecithin. Optimal lysolecithin concentration was found to be 2 to 5 µg per mouse. The phagocytic activity of peritoneal exudate cells from mice was determined by spectral analysis of dioxane extracts of cells after incubation in the presence of polystyrene latex particles. Maximum uptake of latex particles occurred after an incubation period of 1 h. Maximum phagocytic activity was observed in cells harvested 3 days after stimulation with mineral oil and approximately 4 days after stimulation with lysolecithin. Electron microscopy of these cells revealed a similar pattern of phagocytic activity. Latex particles are inert and not metabolized by the cells and their uptake was therefore compared with the phagocytosis of bluetongue virus particles. Electron microscopic studies of the uptake of bluetongue virus showed that although adsorption of the virus on the surface membrane occurred practically no phagocytosis was observed 1 day after stimulation with lysolecithin. Maximum phagocytosis of virus particles occurred 3 to 4 days after stimulation with lysolecithin. en_ZA
dc.identifier.citation Oellermann, RA, Hoogenhout, J & Carter, P 1972, 'Phagocytic activity of peritoneal exudate cells from mice’, Onderstepoort Journal of Veterinary Research, vol. 39, no. 4, pp. 197-203. en_ZA
dc.identifier.issn 0330-2465
dc.language.iso en en_ZA
dc.publisher Published by the Government Printer, Pretoria en_ZA
dc.rights © 1972 ARC- Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). © 2016 University of Pretoria. Department of Library Services(digital). en_ZA
dc.subject Veterinary medicine en_ZA
dc.subject.lcsh Veterinary medicine -- South Africa
dc.title Phagocytic activity of peritoneal exudate cells from mice en_ZA
dc.type Article en_ZA

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