Transmission of jaagsiekte (ovine pulmonary adenomatosis) by means of a permanent epithelial cell line established from affected lungs
Coetzee, S.; Els, H.J.; Verwoerd, Daniel Wynand; De Kock, V.E.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.
An epithelial cell line, designated JS-15. 4, has been established in culture from jaagsiekte lesions
and subcultured in vitro for almost 2 years. It exhibits morphological and other features of transformed
cells and has been shown by electron microscopy to consist of type B ovine alveolar epithelial
cells. Jaagsiekte was successfully transmitted to 3 new-born lambs by the intratracheal injection of
cells following immunosuppressive treatment with either anti-thymocyte immunoglobulin alone or
combined with anti-macrophage immunoglobulin. Incubation periods as short as 10 weeks were
recorded. Evidence was also obtained that natural transmission may result from the inhalation of
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