Carter, P.; Oellermann, R.A.; De Kock, V.E.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.
(Government Printer, Pretoria, 1977)
The optimum conditions for the culture of cells from dissociated spleens were determined. Routinely, 10⁷ cells were seeded per ml of RPMI 1640 medium supplemented with 20% pre-tested foetal calf serum. For the assay of the ...