Browsing 1979 Onderstepoort Journal of Veterinary Research, Volume 46, 1979 by Title

Browsing 1979 Onderstepoort Journal of Veterinary Research, Volume 46, 1979 by Title

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  • A bioassay method for the pheromone(s) of the bont tick Amblyomma hebraeum Koch 
    Brill, J.H.; Solomon, K.R.; Steyn, P.J.J.; Bigalke, R.D.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; Cameron, Colin McKenzie; Gilchrist, Frances M.C. (Published by The Government Printer, Pretoria, 1979)
    A bioassay was developed which enabled the detection of the pheromones of the boot tick (Amblyomma hebraeum Koch) within 2 hours with a reproducibility of 82 ± 13%. Dried, solvent extracted ticks were glued onto the shaved ...
  • Comparative efficacy of six Brucella vaccines in guinea-pigs 
    Cameron, Colin McKenzie; Steyn, P.J.J.; Bigalke, R.D.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; Cameron, Colin McKenzie; Gilchrist, Frances M.C. (Published by The Government Printer, Pretoria, 1979)
    Immunity assays in guinea-pigs based on spleen mass: body mass ratios showed that live vaccines were markedly more effective than inactivated oil adjuvant vaccines in providing protection against challenge with 5 000 ...
  • Composition and evaluation of the efficacy of a Staphylococcus aureus vaccine 
    Cameron, Colin McKenzie; Fuls, W.J.P.; Botha, Wilna F.; Steyn, P.J.J.; Bigalke, R.D.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; Cameron, Colin McKenzie; Gilchrist, Frances M.C. (Published by The Government Printer, Pretoria, 1979)
    An alum-precipitated Staphylococcus aureus vaccine, composed of a formalin-inactivated whole culture of a strain which produces Smith surface antigen and combined with the whole culture of a highly toxigenic strain, was ...
  • Cooperia connochaeti sp. nov. (Nematoda, Trichostrongylidae) from the blue wildebeest, Connochaetes taurinus (Burchell, 1823) 
    Boomker, Jacob Diederik Frederik; Alves, Regina; Horak, Ivan Gerard; Steyn, P.J.J.; Bigalke, R.D.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; Cameron, Colin McKenzie; Gilchrist, Frances M.C. (Published by The Government Printer, Pretoria, 1979)
    A new species of nematode, Cooperia connochaeti, was collected from cross-bred blue and black wildebeest at Krugersdorp (Transvaal), blue wildebeest Connochaetes taurinus (Burchell, 1823) from the Kruger National Park ...
  • Descriptions of the adults and pupa of Culicoides hildae n. sp. from the Republic of South Africa (Diptera : Ceratopogonidae) 
    Cornet, M.; Nevill, E.M.; Steyn, P.J.J.; Bigalke, R.D.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; Cameron, Colin McKenzie; Gilchrist, Frances M.C. (Published by The Government Printer, Pretoria, 1979)
    A new species of biting midge, Culicoides hildae, is described from males, females and pupal exuviae collected in the Orange Free State, Republic of South Africa.
  • The effect of temperature on the frons width in males of Stomoxys calcitrans Linnaeus (Diptera : Muscidae) 
    Sutherland, B.; Steyn, P.J.J.; Bigalke, R.D.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; Cameron, Colin McKenzie; Gilchrist, Frances M.C. (Published by The Government Printer, Pretoria, 1979)
    Laboratory experiments established that males of Stomoxys calcitrans, raised at a high temperature (30°C), had frons widths that were very significantly smaller (P=0,01) than those of males raised at a lower temperature ...
  • An electron microscopic study of intra-erythrocytic stages of Babesia bovis in the brain capillaries of infected splenectomized calves 
    Potgieter, F.T.; Els, H.J.; Steyn, P.J.J.; Bigalke, R.D.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; Cameron, Colin McKenzie; Gilchrist, Frances M. C. (Published by The Government Printer, Pretoria, 1979)
    Splenectomized vaccine donor calves undergoing primary reactions to Babesia bovis infections may develop cerebral babesiosis which leads to death if not treated in time. A brain biopsy was performed on an artificially-infected ...
  • The experimental transmission of Parafilaria bovicola to cattle in South Africa using Musca species (subgenus Eumusca) as intermediate hosts 
    Nevill, E.M.; Steyn, P.J.J.; Bigalke, R.D.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; Cameron, Colin McKenzie; Gilchrist, Frances M.C. (Published by The Government Printer, Pretoria, 1979)
    In controlled experiments in an insect-free stable, cattle became infected with Parafilaria bovicola when Musca lusoria, infected with the larvae of this worm, were allowed to feed on a fresh skin incision, and when infective ...
  • Factors affecting the bluetongue virus neutralizing antibody response and the reaction between virus and antibody 
    Van der Walt, N.T.; Steyn, P.J.J.; Bigalke, R.D.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; Cameron, Colin McKenzie; Gilchrist, Frances M.C. (Published by The Government Printer, Pretoria, 1979)
    A study was made of different aspects of the bluetongue virus neutralizing antibody response and the reaction between the virus and antibody. Optimum neutralization was obtained in a 2 mM Tris-HCl buffer, pH 9,0, at a ...
  • Further characterization of the t-s mutant F207 of bluetongue virus 
    Shipham, S.O.; Steyn, P.J.J.; Bigalke, R.D.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; Cameron, Colin McKenzie; Gilchrist, Frances M.C. (Published by The Government Printer, Pretoria, 1979)
    Temperature-shift experiments verified that the t-s lesion of BTV mutant F207 is expressed late in the replication-cycle, that is, at a stage when all virus components have already been synthesized. All viral polypeptides ...
  • Gastro-intestinal helminths of domestic dogs in the Republic of South Africa 
    Verster, Anna J.M.; Steyn, P.J.J.; Bigalke, R.D.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; Cameron, Colin McKenzie; Gilchrist, Frances M.C. (Published by The Government Printer, Pretoria, 1979)
    Of the gastro-intestinal helminths found in 1 063 dogs from different parts of the country, Dipylidium caninum, the most prevalent cestode, occurred in 19,7% of the animals. Echinococcus granulosus was present in 10 dogs, ...
  • Genetic and physiological characterization of temperature-sensitive mutants of bluetongue virus 
    Shipham, S.O.; De la Rey, Marlene; Steyn, P.J.J.; Bigalke, R.D.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; Cameron, Colin McKenzie; Gilchrist, Frances M.C. (Published by The Government Printer, Pretoria, 1979)
    Complementation studies were carried out, using temperature-sensitive (t-s) mutants of bluetongue virus (BTV). The results proved to be inconclusive as only low indices of complementation were obtained. No discrepancy was ...
  • The haemocytology and histology of the haemopoietic organs of South African fresh water fish. I. The haemopoietic organs of Clarias gariepinus and Sarotherodon mossambicus 
    Boomker, Jacob Diederik Frederik; Steyn, P.J.J.; Bigalke, R.D.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; Cameron, Colin McKenzie; Gilchrist, Frances M.C. (Published by The Government Printer, Pretoria, 1979)
    The haemopoietic organs of the catfish, Clarias gariepinus, and the Mozambique bream, Sarotherodon mossambicus, were studied. In both species the primary haemopoietic organs are the pronephros, the mesonephros and the ...
  • The immune response of horses to tetanus toxoid 
    Jansen, B.C.; Knoetze, P.C.; Steyn, P.J.J.; Bigalke, R.D.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; Cameron, Colin McKenzie; Gilchrist, Frances M.C. (Published by The Government Printer, Pretoria, 1979)
    An intramuscular injection of 8-16 Lf tetanus toxoid in water-in-oil emulsion protected adult horses against tetanus for at least 128 weeks. A booster dose of 8 Lf toxoid in aqueous solution protected them for a further ...
  • Isolation and characterization of antibodies to Clostridium perfringens epsilon toxin from hyperimmune horse serum 
    Worthington, R.W.; Mulders, M.S.G.; Steyn, P.J.J.; Bigalke, R.D.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; Cameron, Colin McKenzie; Gilchrist, Frances M.C. (Published by The Government Printer, Pretoria, 1979)
    Antibodies against epsilon toxin were isolated from hyperimmune horse serum by affinity chromatography. Purified epsilon prototoxin covalently bound to Affigel 202 was used as immunosorbent, and antibodies were eluted with ...
  • The isolation and cultivation of calf rotavirus in the Republic of South Africa 
    Theodoridis, A.; Els, H.J.; Prozesky, Leon.; Steyn, P.J.J.; Bigalke, R.D.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; Cameron, Colin McKenzie; Gilchrist, Frances M.C. (Published by The Government Printer, Pretoria, 1979)
    Calf rotavirus was cultivated and propagated in tissue culture from faeces of 3-week-old calves suffering from severe diarrhoea. Criteria for viral involvement were: production of cytopathic effects in primary foetal calf ...
  • Lymph node pathology in Trypanosoma brucei-infected sheep 
    Barrowman, P.R.; Roos, J.A.; Steyn, P.J.J.; Bigalke, R.D.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; Gilchrist, Frances M.C.; Cameron, Colin McKenzie (Published by The Government Printer, Pretoria, 1979)
    The temporal sequence of morphological changes occurring in the prefemoral lymph nodes of sheep experimentally infected with Trypanosoma brucei was studied at weekly intervals during the course of infection. Histopathological ...
  • Micromorphology of the skin (epidermis, dermis, subcutis) of the dog 
    Schwarz, R.; Le Roux, J.M.W.; Schaller, R.; Neurand, K.; Steyn, P.J.J.; Bigalke, R.D.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; Cameron, Colin McKenzie; Gilchrist, Frances M.C. (Published by The Government Printer, Pretoria, 1979)
    Grossly, the skin of the dog is characterized by varying thicknesses, density of the hair coat and the implantation angle of the hairs. Multiple folds varying in shape are formed by the epidermis and the papillary layer ...
  • The nucleic acid and proteins of epizootic haemorrhagic disease virus 
    Huismans, H. (Henk), 1942-; Bremer, C.W.; Barber, T.L.; Steyn, P.J.J.; Bigalke, R.D.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; Cameron, Colin McKenzie; Gilchrist, Frances M.C. (Published by The Government Printer, Pretoria, 1979)
    Purified epizootic haemorrhagic disease virus (EHDV) was shown to contain 10 double-stranded RNA segments and a double-layered protein capsid with 4 major and 4 minor polypeptides. The virus differed from bluetongue virus ...
  • Parasites of domestic and wild animals in South Africa. XII. Artificial transmission of nematodes from blesbok and impala to sheep, goats and cattle 
    Horak, Ivan Gerard; Steyn, P.J.J.; Bigalke, R.D.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; Gilchrist, Frances M.C.; Cameron, Colin McKenzie (Published by The Government Printer, Pretoria, 1979)
    Sheep were successfully infested artificially with the larvae of Haemonchus contortus, Trichostrongylus axei, Trichostrongylus falculatus and Impalaja nudicollis, cultured from the faeces of blesbok naturally infested with ...