Pneumolysin mediates platelet activation in vitro

Show simple item record Nel, Jan Gert Durandt, Chrisna Mitchell, Timothy J. Feldman, Charles Anderson, Ronald Tintinger, Gregory Ronald 2016-06-23T06:44:47Z 2016-08
dc.description.abstract This study has explored the role of the pneumococcal toxin, pneumolysin (Ply), in activating human platelets. Following exposure to Ply [10–80 nanograms (ng)/ml], platelet activation and cytosolic Ca2+ concentrations were measured flow cytometrically according to the level of expression of CD62P (P-selectin) and spectrofluorimetrically respectively. Exposure to Ply resulted in marked upregulation of expression of platelet CD62P, achieving statistical significance at concentrations of 40 ng/ml and higher (p<0.05), in the setting of increased influx of Ca2+. These potentially pro-thrombotic actions of Ply were attenuated by depletion of Ca2+ from the extracellular medium, or by exposure of the cells to a pneumolysoid devoid of pore-forming activity. These findings are consistent with a mechanism of Plymediated platelet activation involving sub-lytic pore formation, Ca2+ influx, and mobilization of CD62P-expressing α-granules, which, if operative in vivo, may contribute to the pathogenesis of associated acute lung and myocardial injury during invasive pneumococcal disease. en_ZA
dc.description.department Haematology en_ZA
dc.description.department Internal Medicine en_ZA
dc.description.embargo 2017-08-31
dc.description.librarian hb2016 en_ZA
dc.description.uri en_ZA
dc.identifier.citation Nel, JG, Durandt, C, Mitchell, TJ, Feldman, C, Anderson, R & Tintinger, GR 2016, 'Pneumolysin mediates platelet activation in vitro', Lung, vol. 194, no. 4, pp. 589-593. en_ZA
dc.identifier.issn 0341-2040 (print)
dc.identifier.issn 1432-1750 (online)
dc.identifier.other 10.1007/s00408-016-9900-5
dc.language.iso en en_ZA
dc.publisher Springer en_ZA
dc.rights © Springer Science+Business Media New York 2016. The original publication is available at en_ZA
dc.subject Calcium en_ZA
dc.subject CD62P en_ZA
dc.subject Community-acquired pneumonia en_ZA
dc.subject Pneumococcus en_ZA
dc.subject P-selectin en_ZA
dc.subject Streptococcus pneumoniae en_ZA
dc.title Pneumolysin mediates platelet activation in vitro en_ZA
dc.type Postprint Article en_ZA

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