Mating type markers reveal high levels of heterothallism in Leptographium sensu lato

Show simple item record Duong, Tuan A. De Beer, Z. Wilhelm Wingfield, Brenda D. Wingfield, Michael J. 2016-06-22T05:56:44Z 2016-04
dc.description.abstract Species of Leptographium sensu lato (Ophiostomatales, Ascomycetes) are sap-stain fungi vectored by bark beetles (Coleoptera, Scolytinae) and some species cause or are associated with tree diseases. Sexual states have been reported for more than 30 species in this group and these have been treated in the sexual genus Grosmannia. No sexual state is known for at least 59 additional species and these reside in the genus Leptographium. The discovery of sexual states for species of Leptographium relies mainly on the presence of fruiting bodies on host tissue at the time of isolation and/or intensive laboratory mating studies, which commonly have a low levels of success. In this study, markers were developed to diagnose mating-type and to study sexual compatibility of species in Leptographium sensu lato using these markers. To achieve this objective, available mating type sequences for species of Leptographium sensu lato and Ophiostoma were obtained, aligned and used to design primers to amplify MAT genes in Grosmannia and Leptographium species. Using these primers, it was possible to amplify portions of the mating type genes for 42 species and to determine thallism, in many species for the first time. Surprisingly, the results showed that heterothallic and putatively heterothallic species are abundant (39 out of 42 species) in Leptographium sensu lato, and only three species were confirmed to be homothallic. The mating-type markers developed in this study will be useful for future studies concerning mating type and sexual compatibility of species in this genus. en_ZA
dc.description.department Microbiology and Plant Pathology en_ZA
dc.description.embargo 2017-04-30
dc.description.librarian hb2016 en_ZA
dc.description.sponsorship This work was funded by members of the Tree Protection Co-operative Programme (TPCP) and the DST-NRF Centre of Excellence in Tree Health Biotechnology (CTHB) at the Forestry and Agricultural Biotechnology Institute (FABI), the National Research Foundation (NRF) and the University of Pretoria, Pretoria, South Africa. en_ZA
dc.description.uri en_ZA
dc.identifier.citation Duong, TA, De Beer, ZW, Wingfield, BD & Wingfield, MJ 2016, 'Mating type markers reveal high levels of heterothallism in Leptographium sensu lato', Fungal Biology, vol. 120, no. 4, pp. 538-546. en_ZA
dc.identifier.issn 1878-6146 (print)
dc.identifier.issn 1878-6162 (online)
dc.identifier.other 10.1016/j.funbio.2016.01.001
dc.language.iso en en_ZA
dc.publisher Elsevier en_ZA
dc.rights © 2016 The British Mycological Society. Published by Elsevier Ltd. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Fungal Biology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Fungal Biology, vol. 120, no.4, pp. 538-546, 2016. doi : 10.1016/j.funbio.2016.01.001. en_ZA
dc.subject Heterothallism en_ZA
dc.subject Homothallism en_ZA
dc.subject Leptographium en_ZA
dc.subject Grosmannia en_ZA
dc.subject Mating type markers en_ZA
dc.title Mating type markers reveal high levels of heterothallism in Leptographium sensu lato en_ZA
dc.type Postprint Article en_ZA

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