One-stage vs. chromogenic assays in haemophilia A

Show simple item record Potgieter, Joachim Damgaard, Michael Hillarp, Andreas 2016-06-14T07:17:58Z 2016-06-14T07:17:58Z 2015-02
dc.description.abstract Haemophilia A severity is closely correlated to the factor VIII (FVIII) activity, which can be measured in different ways. The original one-stage clotting assay is still the most widely used. The two-stage coagulation assay eliminated many of the drawbacks of the one-stage assay and was further developed into the chromogenic assay, a two-staged test with purified coagulation factors in the first stage, and a FXa-specific chromogenic substrate in the second stage. In many patients with mild or moderate haemophilia A, there is a discrepancy between the one-stage and the two-stage assays. If only the onestage assay is used, some patients will have normal FVIII levels and not be diagnosed as having haemophilia or be considered to have a milder bleeding risk than is the case. Other patients who have normal FVIII activity will be diagnosed as haemophilia A. All haemophilia treatment centre laboratories should have access to both one-stage and chromogenic FVIII:C assays. Appropriate standards should be employed to enable accurate FVIII:C measurement. Different assays to measure inhibitor activity to infused FVIII have been developed since 1959. Inhibitor results based on the one-stage or chromogenic FVIII:C assays are well correlated, but the one-stage assay may be influenced by nonspecific inhibition. en_ZA
dc.description.department Haematology en_ZA
dc.description.librarian hb2016 en_ZA
dc.description.sponsorship Baxter. en_ZA
dc.description.uri en_ZA
dc.identifier.citation Potgieter, J, Damgaard, M & Hillarp, A 2015, 'One-stage vs. chromogenic assays in haemophilia A', European Journal of Haematology, vol. 94, Suppl 77, pp. 38-44. en_ZA
dc.identifier.issn 0902-4441 (print)
dc.identifier.issn 1600-0609 (online)
dc.identifier.other 10.1111/ejh.12500
dc.language.iso en en_ZA
dc.publisher Wiley en_ZA
dc.rights © 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. This is the pre-peer reviewed version of the following article : One-stage vs. chromogenic assays in haemophilia A, European Journal of Haematology, vol. 94, Suppl 77, pp. 38-44, 2015. doi : 10.1111/ejh.12500. The definite version is available at : en_ZA
dc.subject Inhibitors en_ZA
dc.subject Haemophilia en_ZA
dc.subject Chromogenic assays en_ZA
dc.subject One-stage assay en_ZA
dc.title One-stage vs. chromogenic assays in haemophilia A en_ZA
dc.type Postprint Article en_ZA

Files in this item

This item appears in the following Collection(s)

Show simple item record