dc.contributor.author |
Bible, Amber N.
|
|
dc.contributor.author |
Fletcher, Sarah J.
|
|
dc.contributor.author |
Pelletier, Dale A.
|
|
dc.contributor.author |
Schadt, Christopher W.
|
|
dc.contributor.author |
Jawdy, Sara S.
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|
dc.contributor.author |
Weston, David J.
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|
dc.contributor.author |
Engle, Nancy L.
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|
dc.contributor.author |
Tschaplinski, Timothy
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|
dc.contributor.author |
Masyuko, Rachel
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|
dc.contributor.author |
Polisetti, Sneha
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|
dc.contributor.author |
Bohn, Paul W.
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|
dc.contributor.author |
Coutinho, Teresa A.
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|
dc.contributor.author |
Doktycz, Mitchel J.
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|
dc.contributor.author |
Morrell-Falvey, Jennifer L.
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|
dc.date.accessioned |
2016-06-09T07:17:27Z |
|
dc.date.available |
2016-06-09T07:17:27Z |
|
dc.date.issued |
2016-04-18 |
|
dc.description.abstract |
The complex interactions between plants and their microbiome can have a profound effect on the health and productivity of the plant host. A better understanding of the microbial mechanisms that promote plant health and stress tolerance will enable strategies for improving the productivity of economically important plants. Pantoea sp. YR343 is a motile, rod-shaped bacterium isolated from the roots of Populus deltoides that possesses the ability to solubilize phosphate and produce the phytohormone indole-3-acetic acid (IAA). Pantoea sp. YR343 readily colonizes plant roots and does not appear to be pathogenic when applied to the leaves or roots of selected plant hosts. To better understand the molecular mechanisms involved in plant association and rhizosphere survival by Pantoea sp. YR343, we constructed a mutant in which the crtB gene encoding phytoene synthase was deleted. Phytoene synthase is responsible for converting geranylgeranyl pyrophosphate to phytoene, an important precursor to the production of carotenoids. As predicted, the ΔcrtB mutant is defective in carotenoid production, and shows increased sensitivity to oxidative stress. Moreover, we find that the ΔcrtB mutant is impaired in biofilm formation and production of IAA. Finally we demonstrate that the ΔcrtB mutant shows reduced colonization of plant roots. Taken together, these data suggest that carotenoids are important for plant association and/or rhizosphere survival in Pantoea sp. YR343. |
en_ZA |
dc.description.department |
Microbiology and Plant Pathology |
en_ZA |
dc.description.librarian |
am2016 |
en_ZA |
dc.description.sponsorship |
Work at the University of Notre Dame was supported by DOE grant SC0006642 (RM) and by a subcontract from Oak Ridge National Laboratory (SP). |
en_ZA |
dc.description.uri |
http://www.frontiersin.org |
en_ZA |
dc.identifier.citation |
Bible AN, Fletcher SJ, Pelletier DA,
Schadt CW, Jawdy SS, Weston DJ,
Engle LN, Tschaplinski T, Masyuko R,
Polisetti S, Bohn PW, Coutinho TA,
Doktycz MJ and Morrell-Falvey JL
(2016) A Carotenoid-Deficient Mutant
in Pantoea sp. YR343, a Bacteria
Isolated from the Rhizosphere
of Populus deltoides, Is Defective
in Root Colonization.
Front. Microbiol. 7:491.
DOI: 10.3389/fmicb.2016.00491. |
en_ZA |
dc.identifier.issn |
1664-302X |
|
dc.identifier.other |
10.3389/fmicb.2016.00491 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/52917 |
|
dc.language.iso |
en |
en_ZA |
dc.publisher |
Frontiers Research Foundation |
en_ZA |
dc.rights |
© 2016 Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). |
en_ZA |
dc.subject |
Pantoea |
en_ZA |
dc.subject |
Carotenoids |
en_ZA |
dc.subject |
Indole-3-acetic acid |
en_ZA |
dc.subject |
Poplar |
en_ZA |
dc.subject |
Rhizosphere |
en_ZA |
dc.subject |
Zeaxanthin |
en_ZA |
dc.subject |
Plant health |
en_ZA |
dc.subject |
Stress tolerance |
en_ZA |
dc.subject |
CrtB |
en_ZA |
dc.title |
A carotenoid-deficient mutant in Pantoea sp. YR343, a bacteria isolated from the Rhizosphere of Populus deltoides, is defective in root colonization |
en_ZA |
dc.type |
Article |
en_ZA |