Molecular detection and characterisation of mumps virus in cerebrospinal fluid in a Gauteng laboratory

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dc.contributor.author Brauer, Marieke
dc.contributor.author Wolfaardt, Marianne
dc.contributor.author Webber, Lynne M.
dc.contributor.author Taylor, Maureen B.
dc.date.accessioned 2016-06-06T05:59:12Z
dc.date.available 2016-06-06T05:59:12Z
dc.date.issued 2016
dc.description The information contained in this article was presented by Marieke Brauer at the UNIPATH 2014 conference, held on 19-21 September 2014 at the CSIR Convention Centre in Pretoria (no abstract number available). en_ZA
dc.description.abstract The study aimed to determine the presence of mumps virus (MuV) in cerebrospinal fluid (CSF) specimens and to genetically characterise detected MuV strains. A real-time reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the MuV F gene, and characterisation was performed by sequencing of the SH gene. Mumps virus was detected in 1.2% (3/260) of specimens. Phylogenetic analysis of one MuV strain revealed that it clustered with the Jeryl-Lynn and RIT4385 vaccine strains. As far as the authors could ascertain this is the first study to provide viral proof that these vaccine-like strains may be associated with aseptic meningitis. en_ZA
dc.description.department Medical Virology en_ZA
dc.description.librarian am2016 en_ZA
dc.description.uri http://www.sajei.co.za/index.php/SAJEI en_ZA
dc.identifier.citation Brauer, M, Marianne Wolfaardt, M, Webber, LM & Taylor, MB 2016, 'Molecular detection and characterisation of mumps virus in cerebrospinal fluid in a Gauteng laboratory', Southern African Journal of Infectious Diseases, vol. 31, no. 1, pp. 29-31. en_ZA
dc.identifier.issn 2312-0053 (print)
dc.identifier.issn 2313-1810 (online)
dc.identifier.other 10.1080/23120053.2016.1118843
dc.identifier.uri http://hdl.handle.net/2263/52868
dc.language.iso en en_ZA
dc.publisher Medpharm Publications, NISC (Pty) Ltd and Cogent, Taylor & Francis Group en_ZA
dc.rights © 2016 The Author(s). Open Access article distributed under the terms of the Creative Commons License [CC BY-NC-ND 4.0] http://creativecommons.org/licenses/by-nc-nd/4.0. en_ZA
dc.subject Aseptic meningitis en_ZA
dc.subject Central nervous system en_ZA
dc.subject Genotype en_ZA
dc.subject Polymerase chain reaction en_ZA
dc.subject Mumps virus (MuV) en_ZA
dc.subject Cerebrospinal fluid (CSF) en_ZA
dc.subject Real-time reverse transcription-polymerase chain reaction (RT-PCR) en_ZA
dc.title Molecular detection and characterisation of mumps virus in cerebrospinal fluid in a Gauteng laboratory en_ZA
dc.type Article en_ZA


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