First report of an Anabaena Bory strain containing microcystin-LR in a freshwater body in Africa
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Date
Authors
Oberholster, Paul Johan
Jappie, S.
Cheng, P.H.
Botha, A.M.
Matthews, M.W.
Journal Title
Journal ISSN
Volume Title
Publisher
Taylor and Francis
Abstract
In South Africa, little is known about the production of microcystin by the genus Anabaena Bory. In April 2012, during a
cyanobacterial bloom event in Theewaterskloof Dam, Western Cape province, the plankton was sampled on 10 occasions. The
dominant algae belonged to the genus Anabaena, a family of filamentous cyanobacteria known to produce cyanotoxins
such as anatoxin-a, harmful to humans and the aquatic foodweb. The specimens isolated lacked the characteristic akinetes
and/or heterocysts associated with this genus. Therefore the 16S rRNA gene was Sanger sequenced and a maximum parsimony
tree was constructed, confirming its identity as Anabaena ucrainica (Schkorbatow) M. Watanabe. Enzyme-linked
immunosorbent assay (ELISA) confirmed the presence of microcystin-LR in the isolated A. ucrainica field sample, while
PCR analysis and sequencing further confirmed the presence of mcy genes in this species. It was speculated from the
data that prevailing low water-column temperatures and strong gusty winds may have resulted in the lack of akinete or
heterocyst production. The Anabaena strain isolated from Theewaterskloof Dam is the first report of a strain containing
microcystin-LR belonging to this genus in a freshwater body in Africa.
Description
Keywords
Anabaena ucrainica, Heterocysts, Microcystin-LR, Remote sensing, Temperature, Enzyme-linked immunosorbent assay, ELISA, Anabaena Bory strain
Sustainable Development Goals
Citation
PJ Oberholster, S Jappie, PH Cheng, AM Botha & MW Matthews (2015) First report of an Anabaena Bory strain containing microcystin-LR in a freshwater body in Africa, African Journal of Aquatic Science, 40:1, 21-36, DOI:10.2989/16085914.2014.993583.