Excessive pro-inflammatory serum cytokine concentrations in virulent canine Babesiosis

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dc.contributor.author Goddard, Amelia
dc.contributor.author Leisewitz, Andrew L.
dc.contributor.author Kjelgaard-Hansen, Mads
dc.contributor.author Kristensen, Annemarie T.
dc.contributor.author Schoeman, Johan P.
dc.date.accessioned 2016-05-26T09:58:57Z
dc.date.available 2016-05-26T09:58:57Z
dc.date.issued 2015-03-08
dc.description Supporting Information : S1 File. Cytokine Concentrations in Virulent Canine Babesiosis—Leukocyte Data. (PDF) en_ZA
dc.description Supporting Information : S2 File. Cytokine Concentrations in Virulent Canine Babesiosis—APP and Cytokine Data. (PDF) en_ZA
dc.description.abstract Babesia rossi infection causes a severe inflammatory response in the dog, which is the result of the balance between pro- and anti-inflammatory cytokine secretion. The aim of this study was to determine whether changes in cytokine concentrations were present in dogs with babesiosis and whether it was associated with disease outcome. Ninety-seven dogs naturally infected with B. rossi were studied and fifteen healthy dogs were included as controls. Diagnosis of babesiosis was confirmed by polymerase chain reaction and reverse line blot. Blood samples were collected from the jugular vein at admission, prior to any treatment. Cytokine concentrations were assessed using a canine-specific multiplex assay on an automated analyser. Serum concentrations of interleukin (IL)-2, IL-6, IL-8, IL-10, IL-18, granulocyte-macrophage colony stimulating factor (GM-CSF) and monocyte chemotactic protein-1 (MCP-1) were measured. Twelve of the Babesia-infected dogs died (12%) and 85 survived (88%). Babesia-infected dogs were also divided into those that presented within 48 hours fromdisplaying clinical signs, and those that presented more than 48 hours after displaying clinical signs. Cytokine concentrations were compared between the different groups using the Mann-Whitney U test. IL-10 and MCP-1 concentrations were significantly elevated for the Babesia-infected dogs compared to the healthy controls. In contrast, the IL-8 concentration was significantly decreased in the Babesia-infected dogs compared to the controls. Concentrations of IL-6 andMCP-1 were significantly increased in the non-survivors compared to the survivors. Concentrations for IL-2, IL-6, IL-18 and GM-CSF were significantly higher in those cases that presented during the more acute stage of the disease. These findings suggest that a mixed cytokine response is present in dogs with babesiosis caused by B. rossi, and that an excessive pro-inflammatory response may result in a poor outcome. en_ZA
dc.description.department Companion Animal Clinical Studies en_ZA
dc.description.librarian am2016 en_ZA
dc.description.sponsorship Research Development Fund of the University of Pretoria (AG), South African Veterinary Foundation (AG) and National Research Foundation (JPS). en_ZA
dc.description.uri http://www.plosone.org en_ZA
dc.identifier.citation Goddard A, Leisewitz AL, Kjelgaard- Hansen M, Kristensen AT, Schoeman JP (2016) Excessive Pro-Inflammatory Serum Cytokine Concentrations in Virulent Canine Babesiosis. PLoS ONE 11(3): e0150113. DOI: 10.1371/journal.pone.0150113. en_ZA
dc.identifier.issn 1932-6203
dc.identifier.other 10.1371/journal.pone.0150113
dc.identifier.uri http://hdl.handle.net/2263/52759
dc.language.iso en en_ZA
dc.publisher Public Library of Science en_ZA
dc.rights © 2016 Goddard et al. This is an open access article distributed under the terms of the Creative Commons Attribution License. en_ZA
dc.subject Babesia rossi en_ZA
dc.subject Cytokines en_ZA
dc.subject Infection en_ZA
dc.subject Babesia-infected dogs en_ZA
dc.subject.lcsh Dogs -- Diseases en_ZA
dc.title Excessive pro-inflammatory serum cytokine concentrations in virulent canine Babesiosis en_ZA
dc.type Article en_ZA


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