Effect of four medicinal plants on amyloid-p induced neurotoxicity in SH-SY5Y cells
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Date
Authors
Adewusi, Emmanuel Adekanmi
Fouche, Gerda
Steenkamp, Vanessa
Journal Title
Journal ISSN
Volume Title
Publisher
African Networks on Ethnomedicines
Abstract
Amyloid-beta peptide (Aβ) is implicated in the pathogenesis of Alzheimer’s disease (AD), a neurodegenerative
disorder. This study was designed to determine the effect of four medicinal plants used to treat neurodegenerative diseases on Aβ-
induced cell death. Cytotoxicity of the ethanol extracts of the plants was determined against SH-SY5Y (human neuroblastoma)
cells which were untreated, as well as toxically induced with Aβ, using the MTT and neutral red uptake assays. Cell viability was
reduced to 16% when exposed to 20 μM Aβ25-35 for 72 h. The methanol extract of the roots of Ziziphus mucronata Willd., Lannea
schweinfurthii (Engl.) Engl. and Terminalia sericea Burch. ex DC., were the least toxic to the SH-SY5Ycells at the highest
concentration tested (100 μg/ml). All four plants tested were observed to reduce the effects of Aβ-induced neuronal cell death,
indicating that they may contain compounds which may be relevant in the prevention of AD progression.
Description
Keywords
Medicinal plants, Neurodegenerative disorders, Neurotoxicity, Amyloid-beta peptide (Aβ), Alzheimer’s disease (AD), SH-SY5Y cells
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Citation
Adewusia, EA, Fouche, G & Vanessa Steenkamp, V 2013, 'Effect of four medicinal plants on amyloid-p induced neurotoxicity in SH-SY5Y cells', African Journal of Traditional, Complementary and Alternative Medicines (AJTCAM), vol. 10, no. 4, pp. 6-11.