dc.contributor.author |
Jansen van Vuren, Petrus
|
|
dc.contributor.author |
Wiley, Michael
|
|
dc.contributor.author |
Palacios, Gustavo
|
|
dc.contributor.author |
Storm, Nadia
|
|
dc.contributor.author |
McCulloch, Stewart D.
|
|
dc.contributor.author |
Markotter, Wanda
|
|
dc.contributor.author |
Birkhead, Monica
|
|
dc.contributor.author |
Kemp, Alan
|
|
dc.contributor.author |
Paweska, Janusz Tadeusz
|
|
dc.date.accessioned |
2016-05-18T05:52:30Z |
|
dc.date.available |
2016-05-18T05:52:30Z |
|
dc.date.issued |
2016-02-29 |
|
dc.description.abstract |
We report on the isolation of a novel fusogenic orthoreovirus from bat flies (Eucampsipoda
africana) associated with Egyptian fruit bats (Rousettus aegyptiacus) collected in South Africa. Complete
sequences of the ten dsRNA genome segments of the virus, tentatively named Mahlapitsi virus
(MAHLV), were determined. Phylogenetic analysis places this virus into a distinct clade with Baboon
orthoreovirus, Bush viper reovirus and the bat-associated Broome virus. All genome segments of
MAHLV contain a 5' terminal sequence (5'-GGUCA) that is unique to all currently described viruses
of the genus. The smallest genome segment is bicistronic encoding for a 14 kDa protein similar to p14
membrane fusion protein of Bush viper reovirus and an 18 kDa protein similar to p16 non-structural
protein of Baboon orthoreovirus. This is the first report on isolation of an orthoreovirus from an
arthropod host associated with bats, and phylogenetic and sequence data suggests that MAHLV
constitutes a new species within the Orthoreovirus genus. |
en_ZA |
dc.description.department |
Microbiology and Plant Pathology |
en_ZA |
dc.description.librarian |
am2016 |
en_ZA |
dc.description.sponsorship |
The project is jointly funded by the following grants awarded to: Janusz T. Paweska (CDC Global Disease
Detection program, GDD 5U19 GH000571-05/96667), Wanda Markotter (South African National Research
Foundation, Grant UID 91496 and 92524; Poliomyelitis Research Foundation, PRF Grant number 12/14) and
Petrus Jansen van Vuren (South African National Research Foundation, Incentive Funding for Rated Researchers,
Grant UID 85544). |
en_ZA |
dc.description.uri |
http://www.mdpi.com/journal/viruses |
en_ZA |
dc.identifier.citation |
Jansen van Vuren, P, Wiley, M, Palacios, G, Storm, N, McCulloch, S, Markotter, W, Birkhead, M, Kemp, A & Paweska, JT 2016, 'Isolation of a novel fusogenic orthoreovirus from Eucampsipoda africana bat flies in South Africa', Viruses, vol. 8, no. 3, art. #65, pp. 1-25. |
en_ZA |
dc.identifier.issn |
1999-4915 |
|
dc.identifier.other |
10.3390/v8030065 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/52677 |
|
dc.language.iso |
en |
en_ZA |
dc.publisher |
MDPI Publishing |
en_ZA |
dc.rights |
© 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access
article distributed under the terms and conditions of the Creative Commons by Attribution
(CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
en_ZA |
dc.subject |
Fusogenic orthoreovirus |
en_ZA |
dc.subject |
Reoviridae |
en_ZA |
dc.subject |
Nycteribiidae |
en_ZA |
dc.subject |
Bat flies (Eucampsipoda africana) |
en_ZA |
dc.subject |
Egyptian fruit bats (Rousettus aegyptiacus) |
en_ZA |
dc.subject |
South Africa (SA) |
en_ZA |
dc.subject |
Mahlapitsi virus (MAHLV) |
en_ZA |
dc.title |
Isolation of a novel fusogenic orthoreovirus from Eucampsipoda africana bat flies in South Africa |
en_ZA |
dc.type |
Article |
en_ZA |