Additional file 1: Discrimination of specific COI alleles. (A) COI
haplotypes after HaeIII digestion of PCR products: 1-type A, 2-type B, 3-
type C, 4-type D, 5-type E, 6-type E1, 7-marker molecular weight M100;
(B) COI haplotypes after AluI digestion: 1-type A, 2-type B, 3-type C, 4-type
D, 5-type E, 6-type E1, 7-marker molecular weight M50. (TIF 141 kb)
Additional file 2: Example of PCR amplification of specific ACE2 (A)
and CQ11 (B) alleles in Tanger, Morocco. 1–13 - samples, samples 7,
10 - Cx. pipiens form pipiens by both assay. Other samples are hybrids by
ACE2 or CQ11 assays; 14 - marker molecular weight; 15 – Cx.
quinquefasciatus; 16 – Cx.pipiens. (TIF 1408 kb)
Additional file 3: Alignments of ace-2 gene sequences for Cx.
pipiens/quinquefasciatus hybrid collected from Kos, Greece.
Sequences are compared with Cx. pipiens (AY196910) and Cx.
quinquefasciatus (AY196911).“*” Indicates the absence of mutation, “.” -
nucleotide substitutions, “-” indels. (DOCX 12 kb)
Additional file 4: Discrimination of specific wPip alleles based on
ank2 and pk1 markers. (A) three alleles: a (313 bp), b (217, 195, 98 bp)
and c (293, 217 bp) after HinfI digestion of the ank2 PCR products; (B)
three alleles: a/e (903, 430 bp), c (851, 498 bp) and d (497, 251, 107 bp)
after TaqI digestion of the pk1 PCR products; (C) allele a (903, 303,
141 bp) after PstI digestion of the pk1 PCR products. (TIF 557 kb)