Field application of immunoassays for the detection of Mycobacterium bovis infection in the African buffalo (Syncerus caffer)

Show simple item record Van der Heijden, E.M.D.L. (Elisabeth) Jenkins, Akinbowale Olajide Cooper, Dave Rutten, Victor P.M.G. Michel, Anita Luise 2016-05-05T07:24:12Z 2016-05-05T07:24:12Z 2016-01
dc.description.abstract The African buffalo (Syncerus caffer) is considered the most important maintenance host of bovine tuberculosis (BTB) in wildlife in Southern Africa. The diagnosis of Mycobacterium bovis infection in this species mostly relies on the single intradermal comparative tuberculin test (SICTT). As an alternative, the BOVIGAM®1G, an interferon-gamma (IFN- ) release assay, is frequently used. The test performance of cell-mediated immunity (CMI-) and humoral immunity (HI-) based assays for the detection of M. bovis infections in buffaloes was compared to identify the test or test combination that provided the highest sensitivity in the study. Buffaloes were sampled during the annual BTB SICTT testing in the Hluhluwe-iMfolozi-Park (KwaZulu-Natal, South Africa) during June 2013. A total of 35 animals were subjected to the SICTT, 13 of these tested positive and one showed an inconclusive reaction. CMI-based assays (BOVIGAM®1G (B1G) and BOVIGAM®2G (B2G)) as well as a serological assay (IDEXX TB ELISA) were used to further investigate and compare immune responsiveness. Thirteen SICTT positive buffaloes and one inconclusive reactor were slaughtered and a post-mortem (PM) examination was conducted to confirm BTB. Lesions characteristic of BTB were found in 8/14 animals (57.1%). Test results of individual assays were compared with serial and parallel test interpretation and the sensitivity was calculated as a percentage of test positives out of the number of SICTT positive animals with granulomatous lesions (relative sensitivity). The B1G assay showed the highest individual sensitivity (100%; 8/8) followed by the B2G assay (75%; 6/8) and the IDEXX TB ELISA (37.5%; 3/8). Therefore, using in parallel interpretation, any combination with the B1G showed a sensitivity of 100% (8/8), whereas combinations with the B2G showed a 75% sensitivity (6/8).Out of the 21 SICTT negative animals, 7 animals showed responsiveness in the B2G or IDEXX TB ELISA. In conclusion, this study has shown that the BOVIGAM®IFN- assay had the highest test performance. en_ZA
dc.description.department Veterinary Tropical Diseases en_ZA
dc.description.librarian hb2016 en_ZA
dc.description.sponsorship WOTRO Science for Global Development (grant W01.65.321.00). en_ZA
dc.description.uri en_ZA
dc.identifier.citation Van der Heijden, EMDL, Jenkins, AO, Cooper, DV, Rutten, VPMG & Michel, AL 2016, 'Field application of immunoassays for the detection of Mycobacterium bovis infection in the African buffalo (Syncerus caffer)', Veterinary Immunology and Immunopathology, vol. 169, pp. 68-73. en_ZA
dc.identifier.issn 0165-2427 (print)
dc.identifier.issn 1873-2534 (online)
dc.identifier.other 10.1016/j.vetimm.2015.12.003
dc.language.iso en en_ZA
dc.publisher Elsevier en_ZA
dc.rights © 2015 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-NDlicense ( en_ZA
dc.subject Mycobacterium bovis en_ZA
dc.subject African buffalo en_ZA
dc.subject Immunodiagnosis en_ZA
dc.subject Diagnostic test performance en_ZA
dc.subject Bovine tuberculosis en_ZA
dc.subject Single intradermal comparative tuberculin test en_ZA
dc.subject Syncerus caffer
dc.subject BTB
dc.title Field application of immunoassays for the detection of Mycobacterium bovis infection in the African buffalo (Syncerus caffer) en_ZA
dc.type Article en_ZA

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