Characterization of bacterial pathogens in rural and urban irrigation water

Show simple item record Aijuka, Matthew Charimba, George Hugo, Celia J. Buys, E.M. (Elna Maria) 2016-04-22T05:45:43Z 2016-04-22T05:45:43Z 2015-03
dc.description.abstract The study aimed to compare the bacteriological quality of an urban and rural irrigation water source. Bacterial counts, characterization, identification and diversity of aerobic bacteria were determined. Escherichia coli isolated from both sites was subjected to antibiotic susceptibility testing, virulence gene (Stx1/Stx2 and eae) determination and (GTG)5 Rep-PCR fingerprinting. Low mean monthly counts for aerobic spore formers, anaerobic spore formers and Staphylococcus aureus were noted although occasional spikes were observed. The most prevalent bacterial species at both sites were Bacillus spp., E. coli and Enterobacter spp. In addition, E. coli and Bacillus spp. were most prevalent in winter and summer respectively. Resistance to at least one antibiotic was 84% (rural) and 83% (urban). Highest resistance at both sites was to cephalothin and ampicillin. Prevalence of E. coli possessing at least one virulence gene (Stx1/Stx2 and eae) was 15% (rural) and 42% (urban). All (rural) and 80% (urban) of E. coli possessing virulence genes showed antibiotic resistance. Complete genetic relatedness (100%) was shown by 47% of rural and 67% of urban E. coli isolates. Results from this study show that surface irrigation water sources regardless of geographical location and surrounding land-use practices can be reservoirs of similar bacterial pathogens. en_ZA
dc.description.department Food Science en_ZA
dc.description.librarian hb2016 en_ZA
dc.description.sponsorship This study was part of an on-going solicited research project (K5/1875/4) funded by the Water Research Commission and co-funded with the Department of Agriculture, Republic of South Africa. en_ZA
dc.description.uri en_ZA
dc.identifier.citation Aijuka, M, Charimba, G, Hugo, CJ & Buys, EM 2015, 'Characterization of bacterial pathogens in rural and urban irrigation water', Journal of Water and Health, vol. 13, no. 1, pp. 103-117. en_ZA
dc.identifier.issn 1477-8920 (print)
dc.identifier.issn 1996-7829 (online)
dc.identifier.other 10.2166/wh.2014.228
dc.language.iso en en_ZA
dc.publisher IWA Publishing en_ZA
dc.rights © University of Pretoria 2015. The definitive peer-reviewed and edited version of this article is published in Journal of Water and Health, vol.13, no. 1, pp. 103-117, 2015. doi : 10.2166/wh.2014.228, is available at en_ZA
dc.subject Antibiotic resistance en_ZA
dc.subject Bacterial pathogens en_ZA
dc.subject E. coli en_ZA
dc.subject Irrigation water en_ZA
dc.subject (GTG)5-Rep-PCR en_ZA
dc.subject Virulence en_ZA
dc.title Characterization of bacterial pathogens in rural and urban irrigation water en_ZA
dc.type Postprint Article en_ZA

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