The 50% spectrophotometric complement titration end-point (C'H₅₀) was found to be more reproducible than the 100% end-point. The relationship of the 100% end-point (MHD) to the (C'H₅₀) was 1:2, 5. The use of thrombin tubes induced clotting within 2 min and enabled the process of complement preparation to be completed well within 1 hour.
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