Heparinized whole blood, heavily parasitized with Anaplasma marginale, was collected from 3
splenectomized oxen. Buffered lactose peptone (BLP) was added in equal volumes as a stabilizer and
the mixture lyophilized in 2 ml aliquots after rapid freezing. The dried material was reconstituted
with 2 ml sterile water and inoculated without delay. The product remained infective for at least
6 months when stored in an ordinary household deep-freeze unit.
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