Levels of glucose, serum albumin and somatic cells before and during early stages of acute clinical mastitis artificially induced in cows by means of human strains of group-B streptococci (GBS) administered intracisternally

Abstract

The investigation was performed on 3 cows, sampled repeatedly before and during the initial 48h of artificially induced, acute, clinical mastitis. The results of the investigation both augment and support those of earlier work on the levels and significant correlations of glucose, serum albumin and somatic cells in normal and abnormal secretions monitored before and after the usual milking of healthy lactating cows had been suspended. During acute mastitis, udder secretions from artificially infected quarters showed highly significant escalations of somatic cell counts which coincided with equally significant increases on a high and intermediate level of serum albumin values in both the infected and non-infected quarters. Corresponding glucose values fluctuated from 0,07-0,22 and 0,18-0,32 mM in the former and latter quarters respectively. The selective and elevated transfer of serum albumin in otherwise unaffected quarters of acutely mastitic udders suggests rather specific collateral vascular and epithelial changes of unknown nature and magnitude. The data indicate that marked fluctuations of glucose may occur within and between quarters of individual and different cows respectively. Such variations could significantly affect phagocytosis and killing of bacteria challenging the intramammary leucocytic udder barrier before and particularly during manifestation of mastitis. Hence, udder health, although dependent on specific natural defence mechanisms such as the leucocytes and related systems in milk, may depend even more significantly on the supplies of glucose to and within the bovine mammary gland.