There are several stereotypes, prejudices and preconceptions regarding female aviators' abilities and the suitability of women for a career as professional aircraft pilots. This study reports on the development and validation of the Aviation Gender Attitude Questionnaire (AGAQ), which was designed to assess aviators' perceptions about gender-related pilot behavior. A total of 544 South African aircraft pilots participated in the validation of the questionnaire. A four-factor measurement model was established by using exploratory factor analysis and structural equation modeling. Confirmatory Factor Analysis confirmed a good fit between the multifactor solution for the AGAQ and the data. The four factors associated with perceptions about gender-related pilot behavior were Flying Proficiency, Safety Orientation, Flight Confidence and Flight Standards.