Additional file 1: Table S1. Genomic location, Protein ID and GenBank
accession numbers for the sequences used in the present study. (DOC
67 kb)
Additional file 2: Figure S1. The chronogram was inferred using
published calibration time points (see text for detail) and a Bayesian strict
clock approach as implemented in BEAST (Bayesian evolutionary analysis
by sampling trees) v.2 package v.2.2.1 [46]. Horizontal bars mark the
lower and upper time boundaries of the indicated mean age (Million
years ago) estimates of the nodes. The black arrows indicate the four
calibration points, which include the Dothideomycetes crown group
(mean 350 Million years ago [Mya] with 95 % credibility interval [CI] of
273–459) [50], the last common ancestor (LCA) of the Hypocreales (181
Mya with 95 % CI of 150–213) [51], the Clavicipitaceae crown group (117
Mya with 95 % CI of 95–144) [51], as well the Nectriaceae crown group
(125 Mya with 95 % CI of 98–155) [52]. (PDF 227 kb)
Additional file 3: Table S2. List of the putative Fot5 homologs
identified in this study. (DOC 160 kb)
Additional file 4: Figure S2. Maximum likelihood tree of the Fot5 DDD
catalytic domain. This analysis was done using the WAG substitution
model [49] and gamma correction to account for among site rate
variation. Percentage bootstrap support (based on a 1000 repeats, with
cut-off value of 50 %) is indicated at the internodes. Genomic coordinates
of putative Ceratocystis Fot5 homologs are provided in Additional file 3:
Table S2. GenBank accession numbers or sequence identifiers for
previously identified Fot5 homologs are: Fot2 [Genbank:JN624854, F.
oxysporum), Fot5 [Genbank:CAE55867, F. oxysporum], Fot1 [Genbank:
X64799, F. oxysporum], Fot4 [Genbank:AF076632, F. oxysporum], Fot9
[JGI:2517, F. graminearum], Fotyl [Genbank:CAG33729.1 Yarrowia
lipolytica], Molly [Genbank:CAD32687, Parastagonospora nodorum],
Ophio [Genbank:ABG26269, Ophiostoma novo-ulmi], PABRA [Genbank:-
ACY56713, Paracoccidioides brasiliensis], Pixie [Genbank:CAD32689,
Parastagonospora nodorum], Pot2 [Genbank:CAA83918, Magnaporthe
grisea], Pot3 [Genbank:AAC49418, M. grisea], SCSCL [Genbank:
XP001592252, Sclerotinia sclerotiorum], Taf1 [Genbank:AAX83011,
Aspergillus fumigatus], Tan1 [Genbank:U58946, Aspergillus awamori]
USMA [Genbank:UM03882, Ustilago maydis), Flipper [Genbank:
AAB63315, Botryotinia fuckeliana] and Cirt1 [Genbank:XP710204,
Candida albicans]. For the Ceratocystis sequences JS = Ceratocystis
albifundus (green dots), J = Ceratocystis manginecans (red dots) and A
= Ceratocystis fimbriata (blue dots), followed by the genomic position.
(PDF 59 kb)