Intra-serotype SAT2 chimeric foot-and-mouth disease vaccine protects cattle against FMDV challenge

Show simple item record Maree, Francois Frederick Nsamba, Peninah Mutowembwa, Paidamwoyo Rotherham, Lia S. Esterhuysen, Jan J. Scott, Katherine Anne 2016-02-16T05:28:39Z 2015-06
dc.description.abstract The genetic diversity of the three Southern African Territories (SAT) types of foot-and-mouth diseasevirus (FMDV) reflects high antigenic variation, and indications are that vaccines targeting each SAT-specific topotype may be needed. This has serious implications for control of FMD using vaccines as wellas the choice of strains to include in regional antigen banks. Here, we investigated an intra-serotypechimeric virus, vSAT2ZIM14-SAT2, which was engineered by replacing the surface-exposed capsid-codingregion (1B-1D/2A) of a SAT2 genome-length clone, pSAT2, with that of the field isolate, SAT2/ZIM/14/90.The chimeric FMDV produced by this technique was viable, grew to high titres and stably maintained the1B-1D/2A sequence upon passage. Chemically inactivated, oil adjuvanted vaccines of both the chimericand parental immunogens were used to vaccinate cattle. The serological response to vaccination showedthe production of strong neutralizing antibody titres that correlated with protection against homolo-gous FMDV challenge. We also predicted a good likelihood that cattle vaccinated with an intra-serotypechimeric vaccine would be protected against challenge with viruses that caused recent outbreaks insouthern Africa. These results provide support that chimeric vaccines containing the external capsid offield isolates induce protective immune responses in FMD host species similar to the parental vaccine. en_ZA
dc.description.embargo 2016-06-30
dc.description.librarian hb2015 en_ZA
dc.description.sponsorship MSD Animal Health (previously Intervet SPAH) en_ZA
dc.description.uri en_ZA
dc.identifier.citation Maree, FF, Nsamba, P, Mutowembwa, P, Rotherham, LS, Esterhuysen, J & Scott, K 2015, 'Intra-serotype SAT2 chimeric foot-and-mouth disease vaccine protects cattle against FMDV challenge', Vaccine, vol. 33, no. 25, pp. 2909-2916. en_ZA
dc.identifier.issn 0264-410X (print)
dc.identifier.issn 1873-2518 (online)
dc.identifier.other 10.1016/j.vaccine.2015.04.058
dc.language.iso en en_ZA
dc.publisher Elsevier en_ZA
dc.rights © 2015 Elsevier Ltd. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Vaccine. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Vaccine, vol.33, no.25, pp. 2909-2916, 2015. doi : 10.1016/j.vaccine.2015.04.058. en_ZA
dc.subject Intra-serotype chimera en_ZA
dc.subject Chimeric antigen en_ZA
dc.subject Vaccine en_ZA
dc.subject Foot-and-mouth disease virus (FMDV) en_ZA
dc.subject Southern African Territories (SAT) en_ZA
dc.title Intra-serotype SAT2 chimeric foot-and-mouth disease vaccine protects cattle against FMDV challenge en_ZA
dc.type Postprint Article en_ZA

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