Continuous succinic acid fermentation by Escherichia coli KJ122 with cell recycle

Show simple item record Krige, Adolf Nicol, Willie 2016-02-12T12:04:24Z 2015-12
dc.description.abstract High cell densities were obtained by separating the cells with an external hollow fibre filter. Extreme product inhibition at high succinate titres resulted in cell death and subsequent lysis. Accordingly, the highest succinate titre obtained during continuous fermentation was 25 g L−1 at a dilution rate of 0.05 h−1. The highest volumetric productivity of 3 g L−1 h−1 and the highest succinate yield (0.77 g g−1) were obtained at the highest dilution rate (0.15 h−1). The improved yield was caused by increased pyruvate dehydrogenase activity, leading to a decrease in pyruvate and formate excretion and an increase in the reductive flux towards succinate as additional reduction power was produced. The oxidative tricarboxylic acid cycle flux was determined to be minimal, with most of the acetyl coenzyme A (acetyl-CoA) culminating as acetate. Although comparative batch fermentations exhibited a fivefold lower volumetric productivity than the maximum obtained in the cell recycle runs, higher succinate titres (56 g L−1) and yields (0.85 g L−1) were obtained. The higher batch yields were attributed to pyruvate and formate consumption after the termination of cell growth. en_ZA
dc.description.embargo 2016-12-31
dc.description.librarian hb2015 en_ZA
dc.description.sponsorship National Research Foundation (NRF) en_ZA
dc.description.uri en_ZA
dc.identifier.citation Krige, A & Nicol, W 2015, 'Continuous succinic acid fermentation by Escherichia coli KJ122 with cell recycle', Process Biochemistry, vol. 50, no. 12, pp. 2004-2011. en_ZA
dc.identifier.issn 1359-5113 (print)
dc.identifier.issn 1873-3298 (online)
dc.identifier.other 10.1016/j.procbio.2015.09.023
dc.language.iso en en_ZA
dc.publisher Elsevier en_ZA
dc.rights © 2015 Elsevier Ltd. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Process Biochemistry. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Process Biochemistry, vol. 50, no. 12, pp. 2004-2011, 2015. doi : 10.1016/j.procbio.2015.09.023. en_ZA
dc.subject Succinic acid en_ZA
dc.subject E. coli en_ZA
dc.subject Continuous cell recycle en_ZA
dc.subject High-cell-density fermentation en_ZA
dc.subject Metabolic flux analysis en_ZA
dc.title Continuous succinic acid fermentation by Escherichia coli KJ122 with cell recycle en_ZA
dc.type Postprint Article en_ZA

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