Continuous succinic acid production from xylose by Actinobacillus succinogenes

Show simple item record Bradfield, M.F.A. (Michael Ford Alexander) Nicol, Willie 2016-02-10T11:58:59Z 2016-02
dc.description.abstract Continuous, anaerobic fermentations of D-xylose were performed by Actinobacillus succinogenes 130Z in a custom, biofilm reactor at dilution rates of 0.05, 0.10 and 0.30 h-1. Succinic acid yields on xylose (0.55–0.68 g g-1), titres (10.9–29.4 g L-1) and productivities (1.5–3.4 g L-1 h-1) were lower than those of a previous study on glucose, but product ratios (succinic acid/acetic acid = 3.0–5.0 g g-1) and carbohydrate consumption rates were similar. Also, mass balance closures on xylose were up to 18.2 % lower than those on glucose. A modified HPLC method revealed pyruvic acid excretion at appreciable concentrations (1.2–1.9 g L-1) which improved the mass balance closure by up to 16.8 %. Furthermore, redox balances based on the accounted xylose consumed and the excreted metabolites, indicated an overproduction of reducing power. The oxidative pentose phosphate pathway was shown to be a plausible source of the additional reducing power. en_ZA
dc.description.embargo 2017-02-20
dc.description.librarian hb2015 en_ZA
dc.description.sponsorship National Research Foundation (NRF), en_ZA
dc.description.uri en_ZA
dc.identifier.citation Bradfield, MFA & Nicol, W 2016, 'Continuous succinic acid production from xylose by Actinobacillus succinogenes', Bioprocess and Biosystems Engineering, vol. 39, no. 2, pp. 233-244. en_ZA
dc.identifier.issn 1615-7591 (print)
dc.identifier.issn 1615-7605 (online)
dc.identifier.other 10.1007/s00449-015-1507-3
dc.language.iso en en_ZA
dc.publisher Springer en_ZA
dc.rights © Springer-Verlag Berlin Heidelberg 2015. The original publication is available at : , en_ZA
dc.subject Actinobacillus succinogenes en_ZA
dc.subject Continuous fermentation en_ZA
dc.subject Biofilm en_ZA
dc.subject Redox balance en_ZA
dc.subject Succinic acid en_ZA
dc.subject Xylose en_ZA
dc.title Continuous succinic acid production from xylose by Actinobacillus succinogenes en_ZA
dc.type Postprint Article en_ZA

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