dc.contributor.author |
Bradfield, M.F.A. (Michael Ford Alexander)
|
|
dc.contributor.author |
Nicol, Willie
|
|
dc.date.accessioned |
2016-02-10T11:58:59Z |
|
dc.date.issued |
2016-02 |
|
dc.description.abstract |
Continuous, anaerobic fermentations of D-xylose
were performed by Actinobacillus succinogenes 130Z
in a custom, biofilm reactor at dilution rates of 0.05,
0.10 and 0.30 h-1. Succinic acid yields on xylose
(0.55–0.68 g g-1), titres (10.9–29.4 g L-1) and productivities
(1.5–3.4 g L-1 h-1) were lower than those of a
previous study on glucose, but product ratios (succinic
acid/acetic acid = 3.0–5.0 g g-1) and carbohydrate consumption
rates were similar. Also, mass balance closures
on xylose were up to 18.2 % lower than those on glucose.
A modified HPLC method revealed pyruvic acid excretion
at appreciable concentrations (1.2–1.9 g L-1) which
improved the mass balance closure by up to 16.8 %. Furthermore,
redox balances based on the accounted xylose
consumed and the excreted metabolites, indicated an
overproduction of reducing power. The oxidative pentose
phosphate pathway was shown to be a plausible source of
the additional reducing power. |
en_ZA |
dc.description.embargo |
2017-02-20 |
|
dc.description.librarian |
hb2015 |
en_ZA |
dc.description.sponsorship |
National Research Foundation (NRF), |
en_ZA |
dc.description.uri |
http://link.springer.com/journal/449 |
en_ZA |
dc.identifier.citation |
Bradfield, MFA & Nicol, W 2016, 'Continuous succinic acid production from xylose by Actinobacillus succinogenes', Bioprocess and Biosystems Engineering, vol. 39, no. 2, pp. 233-244. |
en_ZA |
dc.identifier.issn |
1615-7591 (print) |
|
dc.identifier.issn |
1615-7605 (online) |
|
dc.identifier.other |
10.1007/s00449-015-1507-3 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/51324 |
|
dc.language.iso |
en |
en_ZA |
dc.publisher |
Springer |
en_ZA |
dc.rights |
© Springer-Verlag Berlin Heidelberg 2015. The original publication is available at : http://link.springer.com/journal/449 , |
en_ZA |
dc.subject |
Actinobacillus succinogenes |
en_ZA |
dc.subject |
Continuous fermentation |
en_ZA |
dc.subject |
Biofilm |
en_ZA |
dc.subject |
Redox balance |
en_ZA |
dc.subject |
Succinic acid |
en_ZA |
dc.subject |
Xylose |
en_ZA |
dc.title |
Continuous succinic acid production from xylose by Actinobacillus succinogenes |
en_ZA |
dc.type |
Postprint Article |
en_ZA |