Abstract:
BACKGROUND : The ability of Frontline Tri-Act®/Frontect®, a topical ectoparasiticide containing fipronil and permethrin
for dogs, to prevent the transmission of Babesia canis as well as Ehrlichia canis was evaluated by infesting dogs with
infected vector ticks.
METHODS : For the Babesia canis study, 16 dogs were randomly allocated to two groups. Eight dogs were treated on
day 0 with a topical spot-on formulation containing 6.76 % w/v fipronil plus 50.48 % w/v permethrin and eight dogs
served as the untreated control group. Dermacentor reticulatus ticks, with a B. canis infection rate ranging between
2 and 10 %, were placed onto dogs on days 7, 14, 21 and 28. In situ tick counts were performed on Days 9, 16 and
23. Ticks were counted and removed on Day 30. Infection of the dogs with B. canis was monitored by rectal temperature
readings, clinical examinations and blood smears as well as PCR and IFA (indirect fluorescent antibody assay).
For the Ehrlichia canis study, another 16 dogs were allocated to two groups. Eight dogs were treated with the fipronil and
permethrin combination on days 0 and 28 and eight dogs served as untreated controls. Rhipicephalus sanguineus ticks,
carrying an infection rate of 13 % for E. canis, were released in the sleeping kennels of the dogs on days 7, 14, 21, 28, 35,
42, 49 and 56. Ticks were counted in situ on the dogs on a weekly basis. All ticks were removed and counted on the final
assessment day 58. Infection of the dogs with E. canis was monitored by rectal temperature, clinical examinations, and
testing of blood samples by PCR, IFA and platelet counts.
RESULTS : B. canis was transmitted by D. reticulatus ticks to all eight untreated control dogs and to one treated dog, which
was confirmed by blood smears, PCR and IFA. E.canis was transmitted by R. sanguineus ticks to all eight untreated control
dogs. Two of the dogs in the treated group were found positive based on PCR and/or IFA.
CONCLUSIONS : Frontline Tri-Act®/Frontect® significantly lowered the risk for dogs to acquire a B. canis infection by 87.5 %
over a challenge period of 28 days. The risk for dogs to acquire E. canis was reduced by 75 % over a period of 56 days.
