Studies on Dictyocaulus filaria. I. Modifications of laboratory procedures

Show simple item record Anderson, P.J.S. Verster, Anna
dc.contributor.editor South Africa. Dept. of Agricultural Technical Services
dc.contributor.editor De Lange, M.
dc.contributor.editor Reinecke, R.K.
dc.contributor.editor Walker, Jane B.
dc.contributor.editor De Kock, V.E.
dc.contributor.editor Howell, P.G. 2015-11-30T07:46:44Z 2015-11-30T07:46:44Z 2015 1971
dc.description The journals have been scanned in colour with a HP 5590 scanner; 600 dpi. Adobe Acrobat v.11 was used to OCR the text and also for the merging and conversion to the final presentation PDF-format. en_ZA
dc.description.abstract Techniques used in a study of the life-cycle of Dictyocaulus filaria (Rudolphi, 1809) are described. First stage larvae migrate from faecal pellets placed in water. Infective larvae are cultured in clean water at room temperature and aerated overnight for 7 days. Sheep are infested per os with infective larvae. At various intervals after infestation they are killed and their organs are cut into small pieces, placed in a modified Baermann apparatus and the parasites allowed to migrate from the tissue into physiological saline at 37°C for 6 to 8 hours. Larvae are collected from lymph drained from the thoracic duct with a catheter. Some of the recovered worms are killed with an iodine solution; others intended for morphological studies are killed by heating to 60°C and preserved in a mixture containing triethanolamine. en_ZA
dc.identifier.citation Anderson, PJS & Verster, A 1971, 'Studies on Dictyocaulus filaria. I. Modifications of laboratory procedures’, Onderstepoort Journal of Veterinary Research, vol. 38, no. 3, pp. 181-4. en_ZA
dc.language.iso en en_ZA
dc.publisher Pretoria : Government Printer en_ZA
dc.rights ©South Africa. Dept. of Agricultural Technical Services (original). ©University of Pretoria. Dept. of Library Services (digital). en_ZA
dc.subject Veterinary medicine en_ZA
dc.subject South Africa en_ZA
dc.subject.lcsh Veterinary medicine -- South Africa
dc.title Studies on Dictyocaulus filaria. I. Modifications of laboratory procedures en_ZA
dc.type Article en_ZA

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