Cardinal temperature differences, determined in vitro, between closely related species and subspecies of pectinolytic bacteria responsible for blackleg and soft rot on potatoes

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dc.contributor.author Du Raan, S.
dc.contributor.author Coutinho, Teresa A.
dc.contributor.author Van der Waals, Jacquie E. (Jacqueline Elise)
dc.date.accessioned 2015-11-20T10:21:12Z
dc.date.issued 2016-02
dc.description.abstract Potato blackleg and soft rot cause major losses and are caused by two bacterial genera, Pectobacterium and Dickeya. Species affecting potatoes are Pectobacterium atrosepticum (Pba), Pectobacterium carotovorum subsp. carotovorum (Pcc), Pectobacterium carotovorum subsp. brasiliense (Pcb), Pectobacterium wasabiae (Pwa), Dickeya dadantii (Dda) and Dickeya solani (Dso). Pathogenicity of these species is dependent on temperature, with each species having its own optimal temperature and temperature range for growth, leading to varying degrees of losses. Pectobacterium atrosepticum, a temperature sensitive species, mainly occurs in temperate climates, Pcc in temperate to tropical, and Dickeya spp. in subtropical environments. The aim of this study was to determine the cardinal growth temperatures for the species responsible for blackleg and soft rot in vitro. Bacterial isolates were incubated in a temperature gradient shaking incubator at 30 different temperatures ranging from ±5 °C to ±56 °C, and growth measured at two set time intervals. Results were statistically analysed using the Gaussian function. The optimal temperature of 31 °C and temperature range of 20 °C to 38 °C for Pectobacterium carotovorum subsp. brasiliense, was similar to those recorded for Pcc. Pectobacterium wasabiae grew at an optimal temperature of 29 °C and range of 20 °C to 34 °C. Higher optimal temperatures of 32 °Cand 34 °C,with ranges of 21 °C to 38 °Cand 23 °C to 41 °C were recorded for Dda and Dso, respectively. The minimal variation in optimal temperatures between different species might be an indication that temperature ranges, rather than optimal temperature, play an important role in disease development. Results for Dso, which has not yet been reported in South Africa, are especially important in light of prevailing temperatures in South African potato production regions. en_ZA
dc.description.embargo 2017-02-20
dc.description.librarian hb2015 en_ZA
dc.description.sponsorship Technology and Human Resources in Industry Programme (THRIP) and Potatoes South Africa en_ZA
dc.description.uri http://link.springer.com/journal/10658 en_ZA
dc.identifier.citation Du Raan, S, Coutinho, TA & Van der Waals, JE 2016. 'Cardinal temperature differences, determined in vitro, between closely related species and subspecies of pectinolytic bacteria responsible for blackleg and soft rot on potatoes', European Journal of Plant Pathology, vol. 144, no. 2, pp. 361-369. en_ZA
dc.identifier.issn 0929-1873 (print)
dc.identifier.issn 1573-8469 (online)
dc.identifier.other 10.1007/s10658-015-0773-x
dc.identifier.uri http://hdl.handle.net/2263/50549
dc.language.iso en en_ZA
dc.publisher Springer en_ZA
dc.rights © Koninklijke Nederlandse Planteziektenkundige Vereniging 2015. The original publication is available at : http://link.springer.comjournal/10658. en_ZA
dc.subject Pectobacterium en_ZA
dc.subject Dickeya en_ZA
dc.subject Growth en_ZA
dc.subject Optimal temperature en_ZA
dc.subject Temperature range en_ZA
dc.subject Pectinolytic bacteria en_ZA
dc.subject Pectobacterium atrosepticum (PBA) en_ZA
dc.subject Pectobacterium carotovorum subsp.carotovorum (PCC) en_ZA
dc.subject Pectobacterium carotovorum subsp. brasiliense (PCB) en_ZA
dc.subject Pectobacterium wasabiae (PWA) en_ZA
dc.subject Dickeya dadantii (DDA) en_ZA
dc.subject Dickeya solani (DSO) en_ZA
dc.title Cardinal temperature differences, determined in vitro, between closely related species and subspecies of pectinolytic bacteria responsible for blackleg and soft rot on potatoes en_ZA
dc.type Postprint Article en_ZA


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