Phylogenetic and expression analysis of the NPR1-like gene family from Persea americana (Mill.)

Show simple item record Backer, Robert Mahomed, Waheed Reeksting, Bianca J. Engelbrecht, Juanita Ibarra-Laclette, Enrique Van der Berg, Noelani 2015-09-28T08:06:30Z 2015-09-28T08:06:30Z 2015-04
dc.description.abstract The NONEXPRESSOR OF PATHOGENESIS-RELATED GENES1 (NPR1) forms an integral part of the salicylic acid (SA) pathway in plants and is involved in cross-talk between the SA and jasmonic acid/ethylene (JA/ET) pathways. Therefore, NPR1 is essential to the effective response of plants to pathogens. Avocado (Persea americana) is a commercially important crop worldwide. Significant losses in production result from Phytophthora root rot, caused by the hemibiotroph, Phytophthora cinnamomi. This oomycete infects the feeder roots of avocado trees leading to an overall decline in health and eventual death. The interaction between avocado and P. cinnamomi is poorly understood and as such limited control strategies exist. Thus uncovering the role of NPR1 in avocado could provide novel insights into the avocado – P. cinnamomi interaction. A total of five NPR1-like sequences were identified. These sequences were annotated using FGENESH and a maximum-likelihood tree was constructed using 34 NPR1-like protein sequences from other plant species. The conserved protein domains and functional motifs of these sequences were predicted. Reverse transcription quantitative PCR was used to analyze the expression of the five NPR1-like sequences in the roots of avocado after treatment with salicylic and jasmonic acid, P. cinnamomi infection, across different tissues and in P. cinnamomi infected tolerant and susceptible rootstocks. Of the five NPR1-like sequences three have strong support for a defensive role while two are most likely involved in development. Significant differences in the expression profiles of these five NPR1-like genes were observed, assisting in functional classification. Understanding the interaction of avocado and P. cinnamomi is essential to developing new control strategies. This work enables further classification of these genes by means of functional annotation and is a crucial step in understanding the role of NPR1 during P. cinnamomi infection. en_ZA
dc.description.librarian am2015 en_ZA
dc.description.sponsorship Student funding was provided by the National Research Foundation and project funding was provided by the Hans Merensky Foundation. en_ZA
dc.description.uri en_ZA
dc.identifier.citation Backer, R, Mahomed, W, Reeksting, BJ, Engelbrecht, J, Ibarra-Laclette, E & Van den Berg, N 2015, 'Phylogenetic and expression analysis of the NPR1-like gene family from Persea americana (Mill.)', Fronties in Plant Science, vol. 6, art. no. 300, pp. 1-15. 10.3389/fpls.2015.00300, en_ZA
dc.identifier.issn 1664-462X
dc.identifier.other 10.3389/fpls.2015.00300
dc.language.iso en en_ZA
dc.publisher Frontiers Research Foundation en_ZA
dc.rights © 2015 Backer, Mahomed, Reeksting, Engelbrecht, Ibarra-Laclette and van den Berg. This is an open-access article distributed under the terms of the Creative Commons Attribution License(CC BY). en_ZA
dc.subject Avocado (Persea americana) en_ZA
dc.subject Phytophthora cinnamomi en_ZA
dc.subject NPR1 en_ZA
dc.subject Expression analysis en_ZA
dc.subject Salicylic acid en_ZA
dc.subject Jasmonic acid en_ZA
dc.subject Pathogenesis-related en_ZA
dc.title Phylogenetic and expression analysis of the NPR1-like gene family from Persea americana (Mill.) en_ZA
dc.type Article en_ZA

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