Elucidating the Ramularia eucalypti species complex

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dc.contributor.author Videira, S.I.R.
dc.contributor.author Groenewald, Johannes Z.
dc.contributor.author Kolecka, A.
dc.contributor.author Van Laren, L.
dc.contributor.author Boekhout, Teun
dc.contributor.author Crous, Pedro W.
dc.date.accessioned 2015-08-28T06:51:54Z
dc.date.available 2015-08-28T06:51:54Z
dc.date.issued 2015
dc.description.abstract The genus Ramularia includes numerous phytopathogenic species, several of which are economically important. Ramularia eucalypti is currently the only species of this genus known to infect Eucalyptus by causing severe leaf-spotting symptoms on this host. However, several isolates identified as R. eucalypti based on morphology and on nrDNA sequence data of the ITS region have recently been isolated from other plant hosts, from environmental samples and also from human clinical specimens. Identification of closely related species based on morphology is often difficult and the ITS region has previously been shown to be unreliable for species level identification in several genera. In this study we aimed to resolve this species-complex by applying a polyphasic approach involving morphology, multi-gene phylogeny and matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS). Six partial genes (ITS, ACT, TEF1-α, HIS3, GAPDH and RPB2) were amplified and sequenced for a total of 44 isolates representing R. eucalypti s.lat. and closely related species. A multi-gene Bayesian phylogenetic analysis and parsimony analysis were performed, and both the resulting trees showed significant support for separation of seven species in R. eucalypti s.lat., including two previously described (R. eucalypti and R. miae), four novel species here described (R. haroldporteri, R. glennii, R. mali and R. plurivora) and one undescribed Ramularia species (sterile). Additionally, Mycosphaerella nyssicola is newly combined in Ramularia as R. nyssicola. Main mass spectra (MSPs) of several R. eucalypti s.lat. strains were generated using MALDI-TOF MS and were compared through a Principal Component Analysis (PCA) dendogram. The PCA dendrogram supported three clades containing R. plurivora, R. glenni /R. mali and R. eucalypti /R. miae. Although the dendrogram separation of species differed from the phylogenetic analysis, the clinically relevant strains were successfully identified by MALDI-TOF MS. en_ZA
dc.description.librarian am2015 en_ZA
dc.description.sponsorship The Dutch Ministry of Education, Culture and Science through an endowment of the FES programme ‘Making the tree of life work’. This publication was also made possible by NPRP grant 5-298-3-086 from the Qatar National Research Fund (a member of the Qatar Foundation) to Teun Boekhout and Anna Kolecka. en_ZA
dc.description.uri http://www.ingentaconnect.com/content/nhn/pimj en_ZA
dc.identifier.citation Videira, SIR, Groenewald, JZ, Kolecka, A, Van Haren, L, Boekhout, T & Crous, PW 2015, 'Elucidating the Ramularia eucalypti species complex', Persoonia, vol. 34, pp. 50-64. en_ZA
dc.identifier.issn 0031-5850
dc.identifier.other 10.3767/003158515X685670
dc.identifier.uri http://hdl.handle.net/2263/49626
dc.language.iso en en_ZA
dc.publisher Naturalis Biodiversity Center and Centraalbureau voor Schimmelcultures en_ZA
dc.rights © 2014-2015 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures en_ZA
dc.subject Mycosphaerellaceae en_ZA
dc.subject Plant pathogen en_ZA
dc.subject Species complex en_ZA
dc.subject Systematics en_ZA
dc.title Elucidating the Ramularia eucalypti species complex en_ZA
dc.type Article en_ZA


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