The anti-inflammatory and antioxidant activity of 25 plant species used traditionally to treat pain in southern African

Show simple item record Adebayo, S.A. Dzoyem, Jean Paul Shai, Leshweni Jeremia Eloff, Jacobus Nicolaas 2015-08-27T05:19:40Z 2015-08-27T05:19:40Z 2015-05-27
dc.description.abstract BACKGROUND : Inflammation is a common risk factor in the pathogenesis of conditions such as infections, arthritis, type 2 diabetes mellitus, obesity and cancer. An ethnobotanical survey of medicinal plants used traditionally to treat inflammation and related disorders such as pain, arthritis and stomach aches in southern Africa led to the selection of 25 plant species used in this study. METHODS : The antioxidant activities of acetone extracts were determined by measuring the free radical scavenging activity and ferric reducing ability, respectively. The anti-inflammatory activities of the extracts were determined by measuring the inhibitory effect of the extracts on the activities of the pro-inflammatory enzyme, lipoxygenase and inducible nitric oxide synthase. RESULTS : Extracts of Peltophorum africanum had good antioxidant activity with IC50 values of 4.67 ± 0.31 μg/mL and 7.71 ± 0.36 μg/mL compared to that of the positive control ascorbic acid (2.92 ± 0.14 μg/mL and 13.57 ± 0.44 μg/mL), using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging and 2,2′-azinobis (3-ethylbenzthiazoline-6-sulphonic acid (ABTS) methods, respectively. The metabolism of linoleic acid to leukotriene derivatives by 15-lipoxygenase (15-LOX) was also inhibited by the crude acetone extracts of Peltophorum africanum (IC50 = 12.42 μg/mL), Zanthoxylum capense (IC50= 14.92 μg/mL) compared to the positive control quercetin (IC50 = 8.75 μg/mL). There was a poor correlation between the flavonoid content and 15-LOX inhibition by the extracts (R2 = 0.05), indicating that flavonoids are not involved in LOX inhibition. Extracts of Clausena anisata, at a concentration of 6.25 μg/mL inhibited nitric oxide production by RAW 264.7 macrophage cell lines in vitro by 96 %. The extracts of Zanthoxylum capense were the least cytotoxic (IC50 > 1000 μg/mL) when the extract toxicity was determined against Vero (African green Monkey) kidney cell lines. CONCLUSION : Some plant species used traditionally to treat pain have reasonable anti-inflammatory activity and flavonoids are probably not involved in this process. en_ZA
dc.description.librarian am2015 en_ZA
dc.description.sponsorship The Tshwane University of Technology, Pretoria, South Africa, and the National Research Foundation (South-Africa). en_ZA
dc.description.uri en_ZA
dc.identifier.citation Adebayo, SA, Dzoyem, JP, Shai, LJ & Eloff, JN 2015, 'The anti-inflammatory and antioxidant activity of 25 plant species used traditionally to treat pain in southern African', BMC Complement​ary and Alternativ​e Medicine, vol. 15, art. #159, pp. 1-10. en_ZA
dc.identifier.issn 1472-6882
dc.identifier.other 10.1186/s12906-015-0669-5
dc.language.iso en en_ZA
dc.publisher BioMed Central en_ZA
dc.rights © 2015 Adebayo et al.; licensee BioMed Central. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. en_ZA
dc.subject Anti-inflammatory en_ZA
dc.subject Medicinal plants en_ZA
dc.subject 15-Lipoxygenase en_ZA
dc.subject Nitric oxide en_ZA
dc.subject Peltophorum africanum en_ZA
dc.title The anti-inflammatory and antioxidant activity of 25 plant species used traditionally to treat pain in southern African en_ZA
dc.type Article en_ZA

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