Cytotoxic effect of a novel synthesized carbazole compound on A549 lung cancer cell line

Show simple item record Molatlhegi, Refilwe P. Phulukdaree, Alisa Anand, Krishnan Gengan, Robert M. Tiloke, Charlette Chuturgoon, A.A. 2015-08-27T05:01:12Z 2015-08-27T05:01:12Z 2015-07-02
dc.description S1 Fig. MTT assay measured in untreated (control) and 1% DMSO (vehicle control) treated A549 cells. The data showed no significant cytotoxicity. en_ZA
dc.description S2 Fig.Western blots showing the effect of ECAP on the expression of Bax, Bcl-2, p53, Nrf2, Hsp70 and SOD. The original western blots which were used for western blot analysis (Fig 6). en_ZA
dc.description.abstract Increased death rates due to lung cancer have necessitated the search for potential novel anticancer compounds such as carbazole derivatives. Carbazoles are aromatic heterocyclic compounds with anticancer, antibacterial and anti-inflammatory activity. The study investigated the ability of the novel carbazole compound (Z)-4-[9-ethyl-9aH-carbazol-3-yl) amino] pent-3-en-2-one (ECAP) to induce cytotoxicity of lung cancer cells and its mechanism of action. ECAP was synthesized as a yellow powder with melting point of 240-247 °C. The 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), lipid peroxidation and comet assays were used to assess the cytotoxic effect of the compound on A549 lung cancer cells. Protein expression was determined using western blots, apoptosis was measured by luminometry (caspase-3/7, -8 and -9) assay and flow cytometry was used to measure phosphatidylserine (PS) externalisation. ECAP induced a p53 mediated apoptosis of lung cancer cells due to a significant reduction in the expression of antioxidant defence proteins (Nrf2 and SOD), Hsp70 (p < 0.02) and Bcl-2 (p < 0.0006), thereby up-regulating reactive oxygen species (ROS) production. This resulted in DNA damage (p < 0.0001), upregulation of Bax expression and caspase activity and induction of apoptosis in lung cancer cells. The results show the anticancer potential of ECAP on lung cancer. en_ZA
dc.description.librarian am2015 en_ZA
dc.description.sponsorship College of Health Sciences, University of KwaZulu-Natal en_ZA
dc.description.uri en_ZA
dc.identifier.citation Molatlhegi RP, Phulukdaree A, Anand K, Gengan RM, Tiloke C, Chuturgoon AA (2015) Cytotoxic Effect of a Novel Synthesized Carbazole Compound on A549 Lung Cancer Cell Line. PLoS ONE 10(7): e0129874. DOI:10.1371/journal.pone.0129874. en_ZA
dc.identifier.issn 1932-6203
dc.identifier.other 10.1371/journal.pone.0129874
dc.language.iso en en_ZA
dc.publisher Public Library of Science en_ZA
dc.rights © 2015 Molatlhegi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License. en_ZA
dc.subject Lung cancer en_ZA
dc.subject Carbazoles en_ZA
dc.subject Yellow powder en_ZA
dc.subject Protein en_ZA
dc.title Cytotoxic effect of a novel synthesized carbazole compound on A549 lung cancer cell line en_ZA
dc.type Article en_ZA

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