The role of HOXB9 and miR-196a in head and neck squamous cell carcinoma

Show simple item record Darda, Lav Hakami, Fahad Morgan, Richard Murdoch, Craig Lambert, Daniel W. Hunter, K.D. (Keith) 2015-08-24T06:50:29Z 2015-08-24T06:50:29Z 2015-04-10
dc.description S1 Fig. Schematic diagram of the putative primary transcript and the length of the PCR product for both the primers utilised in the nested PCR analysis. en_ZA
dc.description S2 Fig. Expression of all HOX genes analysed by qPCR, showing data in the full panel of cell cultures tested, including normal (black), OPM (red) and HNSCC (blue), arranged by group (A-D) and in numerical order. en_ZA
dc.description S3 Fig. Expression of putative miR196a targets suggested form investigation in other cancers, as assessed by qPCR in anti-miR196a transfected D19 and B16 cells, and pre-miR 196a transfected OKF4 cells in panel D. A: Keratin V; B: Anexin A1; C S100A9; D: HOXC8. Only HOXC8 shows significant changes in expression and this only in D19 (p<0.01). The data does not support regulation of KRT5, ANXA1 or S100A9 by miR196a in HNSCC. en_ZA
dc.description S1 Table. Clinical details of the HNSCC and OPM cell lines used in this study. All cell lines are HPV negative. en_ZA
dc.description S2 Table. Clinical and pathological details of the 25 HNSCC samples in the TMA used for HOXB9 IHC. FOM = Floor of mouth, RM = retromolar, BM = buccal mucosa. en_ZA
dc.description S3 Table. Clinical pathological details of HNSCC samples used for Laser capture microdissection FOM = Floor of mouth, RM = retromolar, BM = buccal mucosa. en_ZA
dc.description S4 Table. A full list of qPCR primers used in this study. en_ZA
dc.description S5 Table. Gene ontology enrichment analysis demonstrating significantly enriched GO biological processes on manipulation of miR-196a expression. The total number of significantly differentially expressed genes entered into this analysis was 353. Analysis generated by analysis of the gene list in DAVID ( en_ZA
dc.description.abstract BACKGROUND Previous studies have demonstrated that a number of HOX genes, a family of transcription factors with key roles in early development, are up-regulated in head and neck squamous cell carcinoma (HNSCC) and other cancers. The loci of several Homeobox (HOX) genes also contain microRNAs (miRs), including miR-196a. METHODS Global miR expression and expression of all 39 HOX genes in normal oral keratinocytes (NOKs), oral pre-malignant (OPM) and HNSCC cells was assessed by expressionmicroarray and qPCR and in tissues by immunohistochemistry (IHC) and qPCR of laser microdissected (LCM) tissues. Expression of miR196a and HOXB9 was reduced using anti-miR-196a and siRNA, respectively. Expression microarray profiles of anti-miR196a and pre-miR196a transfected cells were compared to parental cells in order to identify novel targets of miR- 196a. Putative miR196a targets were validated by qPCR and were confirmed as binding to the 3’UTR of miR196a by a dual luciferase reporter assay combined with mutational analysis of the miR-196a binding site. RESULTS miR-196a and HOXB9 are highly expressed in HNSCC compared to NOKs, a pattern also seen in HNSCC tissues by HOXB9 IHC and qPCR of miR-196a in LCM tissue. Knock-down of miR-196a expression decreased HNSCC cell migration, invasion and adhesion to fibronectin, but had no effect on proliferation. Furthermore, knock-down of HOXB9 expression decreased migration, invasion and proliferation but did not alter adhesion. We identified a novel primary mRNA transcript containing HOXB9 and miR196a-1 as predicted from in-silico analysis. Expression array analysis identified a number of miR196a targets, including MAMDC2 and HOXC8.We confirmed that MAMDC2 is a novel miR-196a target using a dual luciferase reporter assay with the effect abolished on mutation of the binding site. CONCLUSIONS These results show that miR-196a and HOXB9 are overexpressed, perhaps co-ordinately, as HNSCC develops and exert a pro-tumourigenic phenotype in HNSCC and OPM cells. en_ZA
dc.description.librarian am2015 en_ZA
dc.description.uri en_ZA
dc.identifier.citation Darda L, Hakami F, Morgan R, Murdoch C, Lambert DW, Hunter KD (2015) The Role of HOXB9 and miR-196a in Head and Neck Squamous Cell Carcinoma. PLoS ONE 10(4): e0122285. DOI: 10.1371/journal.pone.0122285. en_ZA
dc.identifier.issn 1932-6203
dc.identifier.issn 10.1371/journal.pone.0122285
dc.language.iso en en_ZA
dc.publisher Public Library of Science en_ZA
dc.rights © 2015 Darda et al. This is an open access article distributed under the terms of the Creative Commons Attribution License en_ZA
dc.subject Head and neck squamous cell carcinoma (HNSCC) en_ZA
dc.subject Homeobox (HOX) genes en_ZA
dc.subject microRNAs (miRs) en_ZA
dc.subject Normal oral keratinocytes (NOKs) en_ZA
dc.subject Oral pre-malignant (OPM) en_ZA
dc.title The role of HOXB9 and miR-196a in head and neck squamous cell carcinoma en_ZA
dc.type Article en_ZA

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