Development of a real time polymerase chain reaction assay for equine encephalosis virus

Show simple item record Rathogwa, Ntungufhadzeni Maclaughlin Quan, Melvyn Smit, J.Q. Lourens C. Guthrie, Alan John Van Vuuren, Moritz 2015-08-21T11:35:40Z 2015-08-21T11:35:40Z 2014-01
dc.description.abstract Equine encephalosis virus (EEV) is the cause of equine encephalosis. The disease is similar to mild forms of African horse sickness (AHS) and the two diseases are easily confused. Laboratory identification and serotyping of EEV is based on viral isolation in BHK-21 cells and a viral plaque inhibition neutralisation test. These procedures are time-consuming and therefore a more rapid diagnostic assay for EEV that can distinguish EEV from African horse sickness virus (AHSV) infections was developed. The S7 (VP7) gene from 38 EEV isolates representing all seven serotypes was amplified and sequenced. A conserved region at the 5 end of the gene was identified and used to design group-specific EEV primers and a TaqMan® MGBTM hydrolysis probe. The efficiency of the EEV real-time RT-PCR assay was 81%. The assay was specific, as it did not detect any of the nine serotypes of AHSV, nor 24 serotypes of bluetongue virus (BTV) and sensitive, with a 95% limit of detection of 102.9 TCID50/ml blood (95% confidence interval: 102.7 to 103.3). The real-time format was selected because of its convenience, sensitivity and ability to produce results rapidly. en_ZA
dc.description.librarian hb2015 en_ZA
dc.description.sponsorship The Equine Research Centre of the University of Pretoria, Racing South Africa (Pty) Ltd,The Mary Slack and Daughters Foundation and the National Research Foundation, South Africa. en_ZA
dc.description.uri en_ZA
dc.identifier.citation Rathogwa NM, Quan M, Smit JQ, Lourens C, Guthrie AJ & Van Vuuren M 2014, 'Development of a real time polymerase chain reaction assay for equine encephalosis virus', Journal of Virological Methods, vol. 195, pp. 205-210. en_ZA
dc.identifier.issn 0166-0934 (print)
dc.identifier.issn 1879-0984 (online)
dc.identifier.other 10.1016/j.jviromet.2013.10.018
dc.identifier.other N-9080-2014
dc.identifier.other 7004572625
dc.language.iso en en_ZA
dc.publisher Elsevier en_ZA
dc.rights © 2013 Elsevier B.V. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Journal of Virological Methods. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Virological Methods, vol. 195, pp. 205-210, 2014. doi : 10.1016/j.jviromet.2013.10.018. en_ZA
dc.subject Real time RT-PCR en_ZA
dc.subject Diagnostic assay en_ZA
dc.subject Group-specific en_ZA
dc.subject VP7 en_ZA
dc.subject Equine encephalosis virus en_ZA
dc.subject African horse sickness en_ZA
dc.subject African horse sickness virus en_ZA
dc.subject EEV
dc.subject AHS
dc.subject AHSV
dc.subject.lcsh Orbiviruses en_ZA
dc.title Development of a real time polymerase chain reaction assay for equine encephalosis virus en_ZA
dc.type Postprint Article en_ZA

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