dc.contributor.author |
Richards, Sabine
|
|
dc.contributor.author |
Stutzer, Christian
|
|
dc.contributor.author |
Bosman, Anna-Mari
|
|
dc.contributor.author |
Maritz-Olivier, Christine
|
|
dc.date.accessioned |
2015-08-21T11:18:43Z |
|
dc.date.available |
2015-08-21T11:18:43Z |
|
dc.date.issued |
2015-09 |
|
dc.description.abstract |
Managing the spread and load of pathogen-transmitting ticks is an important task worldwide. The cattletick, Rhipicephalus microplus, not only impacts the economy through losses in dairy and meat pro-duction, but also raises concerns for human health in regards to the potential of certain transmittedpathogens becoming zoonotic. However, novel strategies to control R. microplus are hindered by lack ofunderstanding tick biology and the discovery of suitable vaccine or acaricide targets. The importance oftransmembrane proteins as vaccine targets are well known, as is the case in tick vaccines with Bm86 asantigen. In this study, we describe the localization and functional annotation of 878 putative transmem-brane proteins. Thirty proteins could be confirmed in the R. microplus gut using LC-MS/MS analysis andtheir roles in tick biology are discussed. To the best of our knowledge, 19 targets have not been reportedbefore in any proteomics study in various tick species and the possibility of using the identified proteins astargets for tick control are discussed. Although tissue expression of identified putative proteins throughexpansive proteomics is necessary, this study demonstrates the possibility of using bioinformatics forthe identification of targets for further evaluation in tick control strategies. |
en_ZA |
dc.description.embargo |
2016-09-30 |
en_ZA |
dc.description.librarian |
hb2015 |
en_ZA |
dc.description.sponsorship |
Red Meat Research Development Trust, the University of Pretoria Research Development Programme and the Technology and Human Resources for Industry Programme. |
en_ZA |
dc.description.uri |
http://www.elsevier.com/locate/ttbdis |
en_ZA |
dc.identifier.citation |
Richards, SA, Stutzer, C, Bosman, A-M & Maritz-Olivier, C 2015, 'Transmembrane proteins - mining the cattle tick transcriptome', Ticks and Tick-borne Diseases, vol. 6, no. 6, pp. 695-710. |
en_ZA |
dc.identifier.issn |
1877-959X (print) |
|
dc.identifier.issn |
1877-9603 (online) |
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dc.identifier.other |
10.1016/j.ttbdis.2015.06.002 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/49436 |
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dc.language.iso |
en |
en_ZA |
dc.publisher |
Elsevier |
en_ZA |
dc.relation.requires |
Adobe Acrobat Reader |
en |
dc.rights |
© 2015 Elsevier GmbH. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Ticks and Tick-borne Diseases. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Ticks and Tick-borne Diseases, vol. 6, no. 6, pp. 695-710, 2015. doi :10.1016/j.ttbdis.2015.06.002. |
en_ZA |
dc.subject |
Transmembrane proteins |
en_ZA |
dc.subject |
Rhipicephalus microplus |
en_ZA |
dc.subject |
Tick biology |
en_ZA |
dc.subject |
Tick control strategy |
en_ZA |
dc.subject |
Bioinformatics |
en_ZA |
dc.subject |
Proteomics |
en_ZA |
dc.subject.other |
Veterinary science articles SDG-01 |
en_ZA |
dc.subject.other |
SDG-01: No poverty |
|
dc.title |
Transmembrane proteins - mining the cattle tick transcriptome |
en_ZA |
dc.type |
Postprint Article |
en_ZA |