Molecular mechanism of protrusion formation during cell-to-cell spread of Listeria

Show simple item record Ireton, Keith Rigano, Luciano A. Polle, Lilia Schubert, Wolf-Dieter 2015-08-18T08:04:00Z 2015-08-18T08:04:00Z 2014-02
dc.description.abstract The bacterial pathogen Listeria monocytogenes spreads within human tissues using a motility process dependent on the host actin cytoskeleton. Cell-to-cell spread involves the ability of motile bacteria to remodel the host plasma membrane into protrusions, which are internalized by neighboring cells. Recent results indicate that formation of Listeria protrusions in polarized human cells involves bacterial antagonism of a host signaling pathway comprised of the scaffolding protein Tuba and its effectors N-WASP and Cdc42. These three human proteins form a complex that generates tension at apical cell junctions. Listeria relieves this tension and facilitates protrusion formation by secreting a protein called InlC. InlC interacts with a Src Homology 3 (SH3) domain in Tuba, thereby displacing N-WASP from this domain. Interaction of InlC with Tuba is needed for efficient Listeria spread in cultured human cells and infected animals. Recent structural data has elucidated the mechanistic details of InlC/Tuba interaction, revealing that InlC and N-WASP compete for partly overlapping binding surfaces in the Tuba SH3 domain. InlC binds this domain with higher affinity than N-WASP, explaining how InlC is able to disrupt Tuba/N-WASP complexes. en_ZA
dc.description.librarian hb2015 en_ZA
dc.description.sponsorship Grants from the National Institutes of Health (R01AI085072) and the Marsden Fund of the Royal Society of New Zealand (UOO1003), Medical Research Council of South Africa, the National Research Foundation of South Africa and previously by the Helmholtz Centre for Infection Research, Braunschweig, Germany. en_ZA
dc.description.uri en_ZA
dc.identifier.citation Ireton, K, Polle, L, Rigano, LA & Schubert W-D 2014, 'Molecular mechanism of protrusion formation during cell-to-cell spread of Listeria', Frontiers in Cellular and Infection Microbiology, vol. 4, art. no. 21, pp.1-7. en_ZA
dc.identifier.issn 2296-4185 (online)
dc.identifier.other 10.3389/fcimb.2014.00021
dc.language.iso en en_ZA
dc.publisher Frontiers Research Foundation en_ZA
dc.rights © 2014 Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). en_ZA
dc.subject Protrusion en_ZA
dc.subject InlC en_ZA
dc.subject Tuba en_ZA
dc.subject Corticaltension en_ZA
dc.subject SH3 domain en_ZA
dc.subject Structural elucidation en_ZA
dc.subject Listeria monocytogenes en_ZA
dc.subject Cell-to-cell spread en_ZA
dc.title Molecular mechanism of protrusion formation during cell-to-cell spread of Listeria en_ZA
dc.type Article en_ZA

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