Allergen-specific cytokine polarization protects shetland ponies against culicoides obsoletus-induced insect bite hypersensitivity

Show simple item record Meulenbroeks, Chantal Van der Lugt, Jaco J. Van der Meide, N.M.A. Willemse, Ton Rutten, Victor P.M.G. Zaiss, Dietmar M.W. 2015-08-17T11:32:16Z 2015-08-17T11:32:16Z 2015-04-22
dc.description S1 Table. Primers and probes as well as the PCR conditions used for qRT-PCR . en_ZA
dc.description S2 Table. Histological scores from healthy and IBH-affected ponies at different time points after allergen injection. Paraffin-embedded biopsies were cut into sections and stained with either haematoxylin-eosin (HE). Sections were graded according to a semi-quantitative grading system (0 = absent, 1 = minimal, 2 = mild, 3 = moderate, 4 = severe) as previously described 12; average and range between brackets. en_ZA
dc.description.abstract The immunological mechanisms explaining development of an allergy in some individuals and not in others remain incompletely understood. Insect bite hypersensitivity (IBH) is a common, seasonal, IgE-mediated, pruritic skin disorder that affects considerable proportions of horses of different breeds, which is caused by bites of the insect Culicoides obsoletus (C. obsoletus).We investigated the allergen-specific immune status of individual horses that had either been diagnosed to be healthy or to suffer of IBH. Following intradermal allergen injection, skin biopsies were taken of IBH-affected and healthy ponies and cytokine expression was determined by RT-PCR. In addition, allergen-specific antibody titers were measured and cytokine expression of in vitro stimulated, allergen-specific CD4 T-cells was determined. 24 hrs after allergen injection, a significant increase in mRNA expression of the type-2 cytokine IL-4 was observed in the skin of IBH-affected Shetland ponies. In the skin of healthy ponies, however, an increase in IFNγ mRNA expression was found. Analysis of allergen- specific antibody titers revealed that all animals produced allergen-specific antibodies, and allergen-specific stimulation of CD4 T-cells revealed a significant higher percentage of IFNγ-expressing CD4 T-cells in healthy ponies compared to IBH-affected ponies. These data indicate that horses not affected by IBH, in contrast to the so far established dogma, are not immunologically ignorant but have a Th1-skewed allergen-specific immune response that appears to protect against IBH-associated symptoms. To our knowledge this is the first demonstration of a natural situation, in which an allergen-specific immune skewing is protective in an allergic disorder. en_ZA
dc.description.librarian am2015 en_ZA
dc.description.sponsorship The Dutch Technology Foundation (STW-NOW), the Dutch Federation of horse breeding and ALK-Abelló/ Artu Biologicals. en_ZA
dc.description.uri en_ZA
dc.identifier.citation Meulenbroeks C, van der Lugt JJ, van der Meide NMA, Willemse T, Rutten VPMG, Zaiss DMW (2015) Allergen-Specific Cytokine Polarization Protects Shetland Ponies against Culicoides obsoletus-Induced Insect Bite Hypersensitivity. PLoS ONE 10(4): e0122090. DOI: 10.1371/journal.pone.0122090. en_ZA
dc.identifier.issn 1932-6203
dc.identifier.other 10.1371/journal.pone.0122090
dc.language.iso en en_ZA
dc.publisher Public Library of Science en_ZA
dc.relation.requires Adobe Acrobat Reader en
dc.rights © 2015 Meulenbroeks et al. This is an open access article distributed under the terms of the Creative Commons Attribution License en_ZA
dc.subject Allergy en_ZA
dc.subject Insect bite en_ZA
dc.subject Culicoides obsoletus en_ZA
dc.subject Horses en_ZA
dc.subject Insect bite hypersensitivity (IBH) en_ZA
dc.title Allergen-specific cytokine polarization protects shetland ponies against culicoides obsoletus-induced insect bite hypersensitivity en_ZA
dc.type Article en_ZA

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