Molecular and histological association between Candida albicans from oral soft tissue and carious dentine of HIV-positive children

Show simple item record

dc.contributor.author Blignaut, Elaine
dc.contributor.author Van Heerden, W.F.P. (Willem Francois Petrus), 1958-
dc.date.accessioned 2015-08-03T12:34:13Z
dc.date.available 2015-08-03T12:34:13Z
dc.date.issued 2015-10
dc.description.abstract Candida albicans and caries are frequently investigated among healthy and immunosuppressed individuals. The objective of this study was to demonstrate the presence of C. albicans on both oral soft and hard tissue and to investigate, at molecular level, the genetic subtype of the organism from the two oral sites. Tongue swabs and dentine scrapings from 362 HIV-positive children, referred for the extraction of carious primary teeth, were cultured on CHROMagar and identified to species level with ID32C. Histological staining of extracted carious teeth was also done. In patients with positive C. albicans cultures from both the tongue and carious dentine, DNA fingerprinting of such paired isolates was performed, using Southern blot hybridisation with the Ca3 probe. Yeasts were cultured from the tongue of 151 (41.7 %) individuals and 57 (37.7 %) simultaneously yielded positive C. albicans cultures from carious dentine. Nine different yeast spp. were identified from the tongue using the ID32C commercial system, but C. albicans was the only species recovered from carious dentine and histological investigation demonstrated fungal elements penetrated into the dentine and not limited to superficial debris on the floor of the cavity. Twelve of 13 paired isolates of C. albicans revealed identical fingerprinting patterns. The findings from this study demonstrated that in a particular individual, the same genetic subtype of C. albicans was capable of colonising both oral soft tissue and carious dentine. This renders carious teeth a constant source, or reservoir, of potentially infectious agents and, particularly among immunosuppressed individuals, should therefore not be left unattended. en_ZA
dc.description.embargo 2016-10-13 en_ZA
dc.description.librarian hb2015 en_ZA
dc.description.sponsorship MRC grant en_ZA
dc.description.uri http://link.springer.com/journal/11046 en_ZA
dc.identifier.citation Blignaut, E & Van Heerden, WFP 2015, 'Molecular and histological association between Candida albicans from oral soft tissue and carious dentine of HIV-positive children', Mycopathologia, vol. 180, no. 3, pp. 193-201. en_ZA
dc.identifier.issn 0301-486X (print)
dc.identifier.issn 1573-0832 (online)
dc.identifier.other 10.1007/s11046-015-9912-3
dc.identifier.uri http://hdl.handle.net/2263/49247
dc.language.iso en en_ZA
dc.publisher Springer en_ZA
dc.rights © Springer Science+Business Media Dordrecht 2015. The original publication is available at : http://link.springer.comjournal/11046. en_ZA
dc.subject Candida albicans en_ZA
dc.subject DNA fingerprinting en_ZA
dc.subject Deoxyribonucleic acid (DNA) en_ZA
dc.subject Carious teeth en_ZA
dc.subject Human immunodeficiency virus (HIV) en_ZA
dc.subject Paediatric HIV patients en_ZA
dc.subject Children en_ZA
dc.subject Caries en_ZA
dc.subject Fungal colonisation en_ZA
dc.subject Carious dentine en_ZA
dc.title Molecular and histological association between Candida albicans from oral soft tissue and carious dentine of HIV-positive children en_ZA
dc.type Postprint Article en_ZA


Files in this item

This item appears in the following Collection(s)

Show simple item record