The epidemiology of tick-borne haemoparasites as determined by the reverse line blot hybridization assay in an intensively studied cohort of calves in western Kenya

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dc.contributor.author Njiiri, Evalyne Nyawira
dc.contributor.author Bronsvoort, Barend Mark de Clare
dc.contributor.author Collins, Nicola E.
dc.contributor.author Steyn, Helena C.
dc.contributor.author Troskie, Milana
dc.contributor.author Vorster, Ilse
dc.contributor.author Mwangi, Thumbi S.
dc.contributor.author Sibeko-Matjila, K.P. (Kgomotso Penelope)
dc.contributor.author Jennings, Amy
dc.contributor.author Van Wyk, Ilana
dc.contributor.author Mbole-Kariuki, Mary Ndila
dc.contributor.author Kiara, Henry
dc.contributor.author Poole, Jane
dc.contributor.author Hanotte, Olivier
dc.contributor.author Coetzer, Jacobus A.W.
dc.contributor.author Oosthuizen, Marinda C.
dc.contributor.author Woolhouse, Mark E.J.
dc.contributor.author Toye, Philip G.
dc.date.accessioned 2015-07-14T10:03:47Z
dc.date.available 2015-07-14T10:03:47Z
dc.date.issued 2015-05
dc.description.abstract The development of sensitive surveillance technologies using PCR-based detection ofmicrobial DNA, such as the reverse line blot assay, can facilitate the gathering of epidemi-ological information on tick-borne diseases, which continue to hamper the productivityof livestock in many parts of Africa and elsewhere. We have employed a reverse line blotassay to detect the prevalence of tick-borne parasites in an intensively studied cohort ofindigenous calves in western Kenya. The calves were recruited close to birth and monitoredfor the presence of infectious disease for up to 51 weeks. The final visit samples from 453calves which survived for the study period were analyzed by RLB. The results indicated highprevalences of Theileria mutans (71.6%), T. velifera (62.8%), Anaplasma sp. Omatjenne (42.7%),A. bovis (39.9%), Theileria sp. (sable) (32.7%), T. parva (12.9%) and T. taurotragi (8.5%), withminor occurrences of eight other haemoparasites. The unexpectedly low prevalence of thepathogenic species Ehrlichia ruminantium was confirmed by a species-specific PCR targetingthe pCS20 gene region. Coinfection analyses of the seven most prevalent haemoparasitesindicated that they were present as coinfections in over 90% of the cases. The analysesrevealed significant associations between several of the Theileria parasites, in particular T.velifera with Theileria sp. sable and T. mutans, and T. parva with T. taurotragi. There was verylittle coinfection of the two most common Anaplasma species, although they were com-monly detected as coinfections with the Theileria parasites. The comparison of reverse line∗ en_ZA
dc.description.librarian hb2015 en_ZA
dc.description.sponsorship Wellcome Trust (grant no. 079445) through the Infectious Diseases of East African Livestock (IDEAL) project and the Institute of Tropical Medicine (ITM) through the University of Pretoria. en_ZA
dc.description.uri http://www.elsevier.com/locate/vetpar en_ZA
dc.identifier.citation Njiiri, NE, Bronsvoort, BMD, Collins, NE et al 2015, 'The epidemiology of tick-borne haemoparasites as determined by the reverse line blot hybridization assay in an intensively studied cohort of calves in western Kenya', Veterinary Parasitology, vol. 210, no. 1-2, pp. 69-76. en_ZA
dc.identifier.issn 0304-4017 (print)
dc.identifier.issn 1873-2550 (online)
dc.identifier.other 10.1016/j.vetpar.2015.02.020
dc.identifier.other 24332907600
dc.identifier.other O-6028-2014
dc.identifier.other N-8706-2014
dc.identifier.other 7004592997
dc.identifier.other O-6342-2014
dc.identifier.other 7103250386
dc.identifier.uri http://hdl.handle.net/2263/48714
dc.language.iso en en_ZA
dc.publisher Elsevier en_ZA
dc.relation.requires Adobe Acrobat Reader en
dc.rights © 2015 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). en_ZA
dc.subject Theileria en_ZA
dc.subject Anaplasma en_ZA
dc.subject Haemoparasites en_ZA
dc.subject Reverse line blot (RLB) en_ZA
dc.subject Co-infection en_ZA
dc.subject Cattle -- Diseases en_ZA
dc.title The epidemiology of tick-borne haemoparasites as determined by the reverse line blot hybridization assay in an intensively studied cohort of calves in western Kenya en_ZA
dc.type Article en_ZA


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