Detection of equine herpesvirus -4 and physiological stress patterns in young Thoroughbreds consigned to a South African auction sale

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dc.contributor.advisor Schulman, Martin L. en
dc.contributor.coadvisor Page, Patrick Collin en
dc.contributor.coadvisor Guthrie, Alan John
dc.contributor.coadvisor Ganswindt, Andre
dc.contributor.postgraduate Badenhorst, Marcha
dc.date.accessioned 2015-07-02T11:08:32Z
dc.date.available 2015-07-02T11:08:32Z
dc.date.created 2015/04/22 en
dc.date.issued 2015 en
dc.description Dissertation (MSc)--University of Pretoria, 2015. en
dc.description.abstract Commingling of horses from various populations, together with physiological stress associated with transport and confinement at a sales complex, may be associated with detection and transmission of equine herpesvirus type-1 (EHV-1) and -4 (EHV-4). This prospective cohort study aimed to investigate the currently undefined prevalence of EHV-1 and -4 in young Thoroughbreds at an auction sale in South Africa, and associations between clinical signs, physiological stress and viral detection. Ninety, two-year old Thoroughbreds (51 colts, 39 fillies) were consigned from eight farms and sampled at a South African auction sale. The horses were monitored for pyrexia and nasal discharge. Nasal swabs were collected for quantitative polymerase chain reaction (qPCR) assay to detect EHV-1 and -4 and faecal samples were collected for enzyme immunoassay (EIA) to determine faecal glucocorticoid metabolite (FGM) concentrations. EHV-4 nucleic acid was detected in some and EHV-1 nucleic acid in none of the population. Pyrexia and nasal discharge were poor indicators of EHV-4 status. Variation in FGM concentrations was best explained by transportation and preparation for auction. Peaks in EHV-4 detection and increases in FGM concentrations were identified shortly post-arrival and on the first day of auction. Temporal changes in FGM concentrations of horses from individual farms showed two distinct patterns: Pattern A (biphasic peaks) and Pattern B (single peak). It was concluded that sales consignment was associated with some EHV-4 nucleic acid detection and distinctive physiological stress patterns in this population of young Thoroughbreds. en
dc.description.availability Unrestricted en
dc.description.degree MSc en
dc.description.department Companion Animal Clinical Studies en
dc.description.librarian tm2015 en
dc.identifier.citation Badenhorst, M 2015, Detection of equine herpesvirus -4 and physiological stress patterns in young Thoroughbreds consigned to a South African auction sale, MSc Dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/46265> en
dc.identifier.other A2015 en
dc.identifier.other 6507085906
dc.identifier.other G-9856-2014
dc.identifier.other 7006535470
dc.identifier.other J-6375-2013
dc.identifier.other 0000-0001-7729-9918
dc.identifier.uri http://hdl.handle.net/2263/46265
dc.language.iso en en
dc.publisher University of Pretoria en_ZA
dc.rights © 2015 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. en
dc.subject UCTD en
dc.subject Equine herpesvirus
dc.subject Physiological stress
dc.subject Sales consignment
dc.subject Faecal glucocorticoid metabolite (fGCM)
dc.subject Horses
dc.title Detection of equine herpesvirus -4 and physiological stress patterns in young Thoroughbreds consigned to a South African auction sale en
dc.type Dissertation en


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